Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
ADAP / FYB Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G118563-100
Catalog Number: LS-G118563
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human FYB (NM_001465), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- ADAP | FYN-binding protein | FYN-T-binding protein | FYB-120/130 | FYN binding protein | p120/p130 | PRO0823 | SLAP130 | FYB | SLAP-130
- Origin Species
- Human
- NCBI GenBank
- NM_001465
- SwissProt
- O15117
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 90.4 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MAKYNTGGNP TEDVSVNSRP FRVTGPNSSS GIQARKNLFN NQGNASPPAG PSNVPKFGSP61KPPVAVKPSS EEKPDKEPKP PFLKPTGAGQ RFGTPASLTT RDPEAKVGFL KPVGPKPINL121PKEDSKPTFP WPPGNKPSLH SVNQDHDLKP LGPKSGPTPP TSENEQKQAF PKLTGVKGKF181MSASQDLEPK PLFPKPAFGQ KPPLSTENSH EDESPMKNVS SSKGSPAPLG VRSKSGPLKP241AREDSENKDH AGEISSLPFP GVVLKPAASR GGPGLSKNGE EKKEDRKIDA AKNTFQSKIN301QEELASGTPP ARFPKAPSKL TVGGPWGQSQ EKEKGDKNSA TPKQKPLPPL FTLGPPPPKP361NRPPNVDLTK FHKTSSGNST SKGQTSYSTT SLPPPPPSHP ASQPPLPASH PSQPPVPSLP421PRNIKPPFDL KSPVNEDNQD GVTHSDGAGN LDEEQDSEGE TYEDIEASKE REKKREKEEK481KRLELEKKEQ KEKEKKEQEI KKKFKLTGPI QVIHLAKACC DVKGGKNELS FKQGEQIEII541RITDNPEGKW LGRTARGSYG YIKTTAVEID YDSLKLKKDS LGAPSRPIED DQEVYDDVAE601QDDISSHSQS GSGGIFPPPP DDDIYDGIEE EDADDGSTLQ VQEKSNTWSW GILKMLKGKD661DRKKSIREKP KVSDSDNNEG SSFPAPPKQL DMGDEVYDDV DTSDFPVSSA EMSQGTNFGK721AKTEEKDLKK LKKQEKEEKD FRKKFKYDGE IRVLYSTKVT TSITSKKWGT RDLQVKPGES781LEVIQTTDDT KVLCRNEEGK YGYVLRSYLA DNDGEIYDDI ADGCIYDNDTRTRPLEQKLISEEDLAANDILDYKDDDDKV
829 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.