LSBio
Human ACIN1 / Acinus Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

Human ACIN1 / Acinus Protein (Over-Expression Lysate Myc-DDK (Flag))

Expression SystemHuman Embryonic Kidney 293T
Format:Lyophilized
See all ACIN1 antibodies →
$153.00each
Catalog Number: LS-G90095-20

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Overview

Description
This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human Acinus(ACIN1) (NM_001164817) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
ACINUS | ACIN1 | ACN | KIAA0670 | FSAP152
Origin Species
Human
NCBI GenBank
NM_014977
SwissProt
Q9UKV3

Protein

Expression System
Human Embryonic Kidney 293T
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
67.4 kDa
Protein Type
Lysate
Amino Acid Sequence
1MLSESKEGEE KEEVTMDTSE NRPENDVPEP PMPIADQVSN DDRPEGSVED EEKKESSLPK
61SFKRKISVVS ATKGVPAGNS DTEGGQPGRK RRWGASTATT QKKPSISITT ESLKSLIPDI
121KPLAGQEAVV DLHADDSRIS EDETERNGDD GTHDKGLKIC RTVTQVVPAE GQENGQREEE
181EEEKEPEAEP PVPPQVSVEV ALPPPAEHEV KKVTLGDTLT RRSISQQKSG VSITIDDPVR
241TAQVPSPPRG KISNIVHISN LVRPFTLGQL KELLGRTGTL VEEAFWIDKI KSHCFVTYST
301VEEAVATRTA LHGVKWPQSN PKFLCADYAE QDELDYHRGL LVDRPSETKT EEQGIPRPLH
361PPPPPPVQPP QHPRAEQREQ ERAVREQWAE REREMERRER TRSEREWDRD KVREGPRSRS
421RSRDRRRKER AKSKEKKSEK KEKAQEEPPA KLLDDLFRKT KAAPCIYWLP LTDSQIVQKE
481AERAERAKER EKRRKEQEEE EQKEREKEAE RERNRQLERE KRREHSRERD RERERERERD
541RGDRDRDRER DRERGRERDR RDTKRHSRSR SRSTPVRDRG GRR
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

583 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Lyophilized
Formulation
Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4

Storage & Handling

Storage Conditions
Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.