Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
ACIN1 / Acinus Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G128279-100
Catalog Number: LS-G128279
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human Acinus(ACIN1) (NM_001164816), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- ACINUS | ACIN1 | ACN | KIAA0670 | FSAP152
- Origin Species
- Human
- NCBI GenBank
- NM_014977
- SwissProt
- Q9UKV3
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 70.8 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MSPADRCRSA NTIEPATTSS LALFLLLQRD QSSRTRGLPE EKEEVTMDTS ENRPENDVPE61PPMPIADQVS NDDRPEGSVE DEEKKESSLP KSFKRKISVV SATKGVPAGN SDTEGGQPGR121KRRWGASTAT TQKKPSISIT TESLKSLIPD IKPLAGQEAV VDLHADDSRI SEDETERNGD181DGTHDKGLKI CRTVTQVVPA EGQENGQREE EEEEKEPEAE PPVPPQVSVE VALPPPAEHE241VKKVTLGDTL TRRSISQQKS GVSITIDDPV RTAQVPSPPR GKISNIVHIS NLVRPFTLGQ301LKELLGRTGT LVEEAFWIDK IKSHCFVTYS TVEEAVATRT ALHGVKWPQS NPKFLCADYA361EQDELDYHRG LLVDRPSETK TEEQGIPRPL HPPPPPPVQP PQHPRAEQRE QERAVREQWA421EREREMERRE RTRSEREWDR DKVREGPRSR SRSRDRRRKE RAKSKEKKSE KKEKAQEEPP481AKLLDDLFRK TKAAPCIYWL PLTDSQIVQK EAERAERAKE REKRRKEQEE EEQKEREKEA541ERERNRQLER EKRREHSRER DRERERERER DRGDRDRDRE RDRERGRERD RRDTKRHSRS601RSRSTPVRDR GGRRTRTRPLEQKLISEEDLAANDILDYKDDDDKV
614 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.