ACE / CD143 Antibody
Catalog Number: LS-C405399
4 sizes / from $260.00
Antibody: Rabbit Polyclonal
Applications: Enzyme-Linked Immunosorbent Assay, Western Blot
Reactivity: Human, Mouse, Rat
Format: Liquid
Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
Human ACE / CD143 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293T |
| Format: | Lyophilized |
$220.00each
Catalog Number: LS-G74508-20
Catalog Number: LS-G74508
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- Description
- This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human Angiotensin Converting Enzyme 1(ACE) (NM_001178057) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- ACE | ACE1 | Angiotensin-converting enzyme | CD143 | CD143 antigen | Carboxycathepsin | DIPEPTIDYL CARBOXYPEPTIDASE | DCP | Dipeptidyl carboxypeptidase I | ICH | Kininase II | MVCD3 | Peptidyl-dipeptidase A | Peptidase P | DCP1 | Dipeptidyl carboxypeptidase 1 | Testicular ECA
- Origin Species
- Human
- NCBI GenBank
- NM_000789
- SwissProt
- P12821
Protein
- Expression System
- Human Embryonic Kidney 293T
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 79.1 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MGQGWATAGL PSLLFLLLCY GHPLLVPSQE ASQQVTVTHG TSSQATTSSQ TTTHQATAHQ61TSAQSPNLVT DEAEASKFVE EYDRTSQVVW NEYAEANWNY NTNITTETSK ILLQKNMQIA121NHTLKYGTQA RKFDVNQLQN TTIKRIIKKV QDLERAALPA QELEEYNKIL LDMETTYSVA181TVCHPNGSCL QLEPDLTNVM ATSRKYEDLL WAWEGWRDKA GRAILQFYPK YVELINQAAR241LNGYVDAGDS WRSMYETPSL EQDLERLFQE LQPLYLNLHA YVRRALHRHY GAQHINLEGP301IPAHLLGNMW AQTWSNIYDL VVPFPSAPSM DTTEAMLKQG WTPRRMFKEA DDFFTSLGLL361PVPPEFWNKS MLEKPTDGRE VVCHASAWDF YNGKDFRIKQ CTTVNLEDLV VAHHEMGHIQ421YFMQYKDLPV ALREGANPGF HEAIGDVLAL SVSTPKHLHS LNLLSSEGGS DEHDINFLMK481MALDKIAFIP FSYLVDQWRW RVFDGSITKE NYNQEWWSLR LKYQGLCPPV PRTQGDFDPG541AKFHIPSSVP YIRTAMKLGF SRPWPEAMQL ITGQPNMSAS AMLSYFKPLL DWLRTENELH601GEKLGWPQYN WTPNSARSEG PLPDSGRVSF LGLDLDAQQA RVGQWLLLFL GIALLVATLG661LSQRLFSIRH RSLHRHSHGP QFGSEVELRH STRTRPLEQKLISEEDLAANDILDYKDDDDKV
691 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Lyophilized
- Formulation
- Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4
Storage & Handling
- Storage Conditions
- Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.
