Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
ACE / CD143 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G130169-100
Catalog Number: LS-G130169
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human Angiotensin Converting Enzyme 1(ACE) (NM_001178057), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- ACE | ACE1 | Angiotensin-converting enzyme | CD143 | CD143 antigen | Carboxycathepsin | DIPEPTIDYL CARBOXYPEPTIDASE | DCP | Dipeptidyl carboxypeptidase I | ICH | Kininase II | MVCD3 | Peptidyl-dipeptidase A | Peptidase P | DCP1 | Dipeptidyl carboxypeptidase 1 | Testicular ECA
- Origin Species
- Human
- NCBI GenBank
- NM_000789
- SwissProt
- P12821
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 79.1 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MGQGWATAGL PSLLFLLLCY GHPLLVPSQE ASQQVTVTHG TSSQATTSSQ TTTHQATAHQ61TSAQSPNLVT DEAEASKFVE EYDRTSQVVW NEYAEANWNY NTNITTETSK ILLQKNMQIA121NHTLKYGTQA RKFDVNQLQN TTIKRIIKKV QDLERAALPA QELEEYNKIL LDMETTYSVA181TVCHPNGSCL QLEPDLTNVM ATSRKYEDLL WAWEGWRDKA GRAILQFYPK YVELINQAAR241LNGYVDAGDS WRSMYETPSL EQDLERLFQE LQPLYLNLHA YVRRALHRHY GAQHINLEGP301IPAHLLGNMW AQTWSNIYDL VVPFPSAPSM DTTEAMLKQG WTPRRMFKEA DDFFTSLGLL361PVPPEFWNKS MLEKPTDGRE VVCHASAWDF YNGKDFRIKQ CTTVNLEDLV VAHHEMGHIQ421YFMQYKDLPV ALREGANPGF HEAIGDVLAL SVSTPKHLHS LNLLSSEGGS DEHDINFLMK481MALDKIAFIP FSYLVDQWRW RVFDGSITKE NYNQEWWSLR LKYQGLCPPV PRTQGDFDPG541AKFHIPSSVP YIRTAMKLGF SRPWPEAMQL ITGQPNMSAS AMLSYFKPLL DWLRTENELH601GEKLGWPQYN WTPNSARSEG PLPDSGRVSF LGLDLDAQQA RVGQWLLLFL GIALLVATLG661LSQRLFSIRH RSLHRHSHGP QFGSEVELRH STRTRPLEQKLISEEDLAANDILDYKDDDDKV
691 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.