LSBio
Human AATK / AATYK Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

Human AATK / AATYK Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293T
Format:Lyophilized
See all AATK antibodies →
$220.00each
Catalog Number: LS-G88198-20

For bulk orders or custom formulations:

Request a Quote

Overview

Description
This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human AATK (NM_001080395) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
AATYK | AATK | AATYK1 | CDK5-binding protein | Lemur tyrosine kinase 1 | p35-binding protein | p35BP | KIAA0641 | LMR1 | LMTK1
Origin Species
Human
NCBI GenBank
AB014541
SwissProt
Q6ZMQ8

Protein

Expression System
Human Embryonic Kidney 293T
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
148.28 kDa
Protein Type
Lysate
Amino Acid Sequence
1MTQNGPRSWL CPPVPLAWQV TVLDVAFLPF LKATFSSPFN LPEPSEVATG PINQTRLAPG
61QQALKEFWEL RRWCGHQVKV QGCWGRWRWQ EFENAEGDEY AADLAQGSPA TAAQNGPDVY
121VLPLTEVSLP MAKQPGRSVQ LLKSTDVGRH SLLYLKEIGR GWFGKVFLGE VNSGISSAQV
181VVKELQASAS VQEQMQFLEE VQPYRALKHS NLLQCLAQCA EVTPYLLVME FCPLGDLKGY
241LRSCRVAESM APDPRTLQRM ACEVACGVLH LHRNNFVHSD LALRNCLLTA DLTVKIGDYG
301LAHCKYREDY FVTADQLWVP LRWIAPELVD EVHSNLLVVD QTKSGNVWSL GVTIWELFEL
361GTQPYPQHSD QQVLAYTVRE QQLKLPKPQL QLTLSDRWYE VMQFCWLQPE QRPTAEEVHL
421LLSYLCAKGA TEAEEEFERR WRSLRPGGGG VGPGPGAAGP MLGGVVELAA ASSFPLLEQF
481AGDGFHADGD DVLTVTETSR GLNFEYKWEA GRGAEAFPAT LSPGRTARLQ ELCAPDGAPP
541GVVPVLSAHS PSLGSEYFIR LEEAAPAAGH DPDCAGCAPS PPATADQDDD SDGSTAASLA
601MEPLLGHGPP VDVPWGRGDH YPRRSLARDP LCPSRSPSPS AGPLSLAEGG AEDADWGVAA
661FCPAFFEDPL GTSPLGSSGA PPLPLTGEDE LEEVGARRAA QRGHWRSNVS ANNNSGSRCP
721ESWDPVSAGG HAEGCPSPKQ TPRASPEPGY PGEPLLGLQA ASAQEPGCCP GLPHLCSAQG
781LAPAPCLVTP SWTETASSGG DHPQAEPKLA TEAEGTTGPR LPLPSVPSPS QEGAPLPSEE
841ASAPDAPDAL PDSPTPATGG EVSAIKLASA LNGSSSSPEV EAPSSEDEDT AEATSGIFTD
901TSSDGLQARR PDVVPAFRSL QKQVGTPDSL DSLDIPSSAS DGGYEVFSPS ATGPSGGQPR
961ALDSGYDTEN YESPEFVLKE AQEGCEPQAF AELASEGEGP GPETRLSTSL SGLNEKNPYR
1021DSAYFSDLEA EAEATSGPEK KCGGDRAPGP ELGLPSTGQP SEQVCLRPGV SGEAQGSGPG
1081EVLPPLLQLE GSSPEPSTCP SGLVPEPPEP QGPAKVRPGP SPSCSQFFLL TPVPLRSEGN
1141SSEFQGPPGL LSGPAPQKRM GGPGTPRAPL RLALPGLPAA LEGRPEEEEE DSEDSDESDE
1201ELRCYSVQEP SEDSEEEAPA VPVVVAESQS ARNLRSLLKM PSLLSETFCE DLERKKKAVS
1261FFDDVTVYLF DQESPTRELG EPFPGAKESP PTSLRGSPGS PSAPNRPQQA DGSPNGSTAE
1321EGGGFAWDDD FPLMTAKAAF AMALDPAAPA PAAPTPTPAP FSRFTVSPAP TSRFSITHVS
1381DSDAESKRGP EAGAGGESKE A
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

1401 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Lyophilized
Formulation
Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4

Storage & Handling

Storage Conditions
Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.