Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
AATK / AATYK Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G120183-100
Catalog Number: LS-G120183
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human AATK (NM_001080395), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- AATYK | AATK | AATYK1 | CDK5-binding protein | Lemur tyrosine kinase 1 | p35-binding protein | p35BP | KIAA0641 | LMR1 | LMTK1
- Origin Species
- Human
- NCBI GenBank
- AB014541
- SwissProt
- Q6ZMQ8
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 148.28 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MTQNGPRSWL CPPVPLAWQV TVLDVAFLPF LKATFSSPFN LPEPSEVATG PINQTRLAPG61QQALKEFWEL RRWCGHQVKV QGCWGRWRWQ EFENAEGDEY AADLAQGSPA TAAQNGPDVY121VLPLTEVSLP MAKQPGRSVQ LLKSTDVGRH SLLYLKEIGR GWFGKVFLGE VNSGISSAQV181VVKELQASAS VQEQMQFLEE VQPYRALKHS NLLQCLAQCA EVTPYLLVME FCPLGDLKGY241LRSCRVAESM APDPRTLQRM ACEVACGVLH LHRNNFVHSD LALRNCLLTA DLTVKIGDYG301LAHCKYREDY FVTADQLWVP LRWIAPELVD EVHSNLLVVD QTKSGNVWSL GVTIWELFEL361GTQPYPQHSD QQVLAYTVRE QQLKLPKPQL QLTLSDRWYE VMQFCWLQPE QRPTAEEVHL421LLSYLCAKGA TEAEEEFERR WRSLRPGGGG VGPGPGAAGP MLGGVVELAA ASSFPLLEQF481AGDGFHADGD DVLTVTETSR GLNFEYKWEA GRGAEAFPAT LSPGRTARLQ ELCAPDGAPP541GVVPVLSAHS PSLGSEYFIR LEEAAPAAGH DPDCAGCAPS PPATADQDDD SDGSTAASLA601MEPLLGHGPP VDVPWGRGDH YPRRSLARDP LCPSRSPSPS AGPLSLAEGG AEDADWGVAA661FCPAFFEDPL GTSPLGSSGA PPLPLTGEDE LEEVGARRAA QRGHWRSNVS ANNNSGSRCP721ESWDPVSAGG HAEGCPSPKQ TPRASPEPGY PGEPLLGLQA ASAQEPGCCP GLPHLCSAQG781LAPAPCLVTP SWTETASSGG DHPQAEPKLA TEAEGTTGPR LPLPSVPSPS QEGAPLPSEE841ASAPDAPDAL PDSPTPATGG EVSAIKLASA LNGSSSSPEV EAPSSEDEDT AEATSGIFTD901TSSDGLQARR PDVVPAFRSL QKQVGTPDSL DSLDIPSSAS DGGYEVFSPS ATGPSGGQPR961ALDSGYDTEN YESPEFVLKE AQEGCEPQAF AELASEGEGP GPETRLSTSL SGLNEKNPYR1021DSAYFSDLEA EAEATSGPEK KCGGDRAPGP ELGLPSTGQP SEQVCLRPGV SGEAQGSGPG1081EVLPPLLQLE GSSPEPSTCP SGLVPEPPEP QGPAKVRPGP SPSCSQFFLL TPVPLRSEGN1141SSEFQGPPGL LSGPAPQKRM GGPGTPRAPL RLALPGLPAA LEGRPEEEEE DSEDSDESDE1201ELRCYSVQEP SEDSEEEAPA VPVVVAESQS ARNLRSLLKM PSLLSETFCE DLERKKKAVS1261FFDDVTVYLF DQESPTRELG EPFPGAKESP PTSLRGSPGS PSAPNRPQQA DGSPNGSTAE1321EGGGFAWDDD FPLMTAKAAF AMALDPAAPA PAAPTPTPAP FSRFTVSPAP TSRFSITHVS1381DSDAESKRGP EAGAGGESKE ATRTRPLEQKLISEEDLAANDILDYKDDDDKV
1401 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.