Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
HTR2A / 5-HT2A Receptor Protein (Over-Expression Lysate Myc-DDK (Flag))
ProteinLSBio Guarantee
| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G128095-100
Catalog Number: LS-G128095
For bulk orders or custom formulations:
Request a QuoteOverview
- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human 5 HT 2A(HTR2A) (NM_001165947), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- 5-HT2A | 5-HT-2A | 5-HT2a receptor | 5HT2A Receptor | 5-HT-2 | 5-HT2A/2C | 5-HT2A/2C receptor | 5-HT2 receptor | HTR2 | HTR2A | Serotonin 5-HT-2A receptor | Serotonin receptor 2A | Serotonin 2a receptor
- Origin Species
- Human
- NCBI GenBank
- NM_000621
- SwissProt
- P28223
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 43.8 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MQFLKSAKQK PNYYHIMLVE DQEEGTLHQF NYCERCSESQ NNKCISCVDP EDKWYRWPLP61SKLCAVWIYL DVLFSTASIM HLCAISLDRY VAIQNPIHHS RFNSRTKAFL KIIAVWTISV121GISMPIPVFG LQDDSKVFKE GSCLLADDNF VLIGSFVSFF IPLTIMVITY FLTIKSLQKE181ATLCVSDLGT RAKLASFSFL PQSSLSSEKL FQRSIHREPG SYTGRRTMQS ISNEQKACKV241LGIVFFLFVV MWCPFFITNI MAVICKESCN EDVIGALLNV FVWIGYLSSA VNPLVYTLFN301KTYRSAFSRY IQCQYKENKK PLQLILVNTI PALAYKSSQL QMGQKKNSKQ DAKTTDNDCS361MVALGKQHSE EASKDNSDGV NEKVSCVTRTRPLEQKLISEEDLAANDILDYKDDDDKV
387 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.