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HKDC1 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

HKDC1 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$725.00each
Catalog Number: LS-G109574-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human HKDC1 (NM_025130), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
HKDC1 | Putative hexokinase HKDC1 | Hexokinase domain containing 1
Origin Species
Human
NCBI GenBank
NM_025130
SwissProt
Q2TB90

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
102.3 kda
Protein Type
Lysate
Amino Acid Sequence
1MFAVHLMAFY FSKLKEDQIK KVDRFLYHMR LSDDTLLDIM RRFRAEMEKG LAKDTNPTAA
61VKMLPTFVRA IPDGSENGEF LSLDLGGSKF RVLKVQVAEE GKRHVQMESQ FYPTPNEIIR
121GNGTELFEYV ADCLADFMKT KDLKHKKLPL GLTFSFPCRQ TKLEEGVLLS WTKKFKARGV
181QDTDVVSRLT KAMRRHKDMD VDILALVNDT VGTMMTCAYD DPYCEVGVII GTGTNACYME
241DMSNIDLVEG DEGRMCINTE WGAFGDDGAL EDIRTEFDRE LDLGSLNPGK QLFEKMISGL
301YLGELVRLIL LKMAKAGLLF GGEKSSALHT KGKIETRHVA AMEKYKEGLA NTREILVDLG
361LEPSEADCIA VQHVCTIVSF RSANLCAAAL AAILTRLREN KKVERLRTTV GMDGTLYKIH
421PQYPKRLHKV VRKLVPSCDV RFLLSESGST KGAAMVTAVA SRVQAQRKQI DRVLALFQLT
481REQLVDVQAK MRAELEYGLK KKSHGLATVR MLPTYVCGLP DGTEKGKFLA LDLGGTNFRV
541LLVKIRSGRR SVRMYNKIFA IPLEIMQGTG EELFDHIVQC IADFLDYMGL KGASLPLGFT
601FSFPCRQMSI DKGTLIGWTK GFKATDCEGE DVVDMLREAI KRRNEFDLDI VAVVNDTVGT
661MMTCGYEDPN CEIGLIAGTG SNMCYMEDMR NIEMVEGGEG KMCINTEWGG FGDNGCIDDI
721RTRYDTEVDE GSLNPGKQRY EKMTSGMYLG EIVRQILIDL TKQGLLFRGQ ISERLRTRGI
781FETKFLSQIE SDRLALLQVR RILQQLGLDS TCEDSIVVKE VCGAVSRRAA QLCGAGLAAI
841VEKRREDQGL EHLRITVGVD GTLYKLHPHF SRILQETVKE LAPRCDVTFM LSEDGSGKGA
901ALITAVAKRL QQAQKEN
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

917 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

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To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.