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HECTD3 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

HECTD3 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all HECTD3 antibodies →
$725.00each
Catalog Number: LS-G109884-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human HECTD3 (NM_024602), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
HECT domain containing 3 | RP11-69J16.1 | HECTD3
Origin Species
Human
NCBI GenBank
NM_024602
SwissProt
Q5T447

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
97.6 kDa
Protein Type
Lysate
Amino Acid Sequence
1MAGPGPGAVL ESPRQLLGRV RFLAEAARSL RAGRPLPAAL AFVPREVLYK LYKDPAGPSR
61VLLPVWEAEG LGLRVGAAGP APGTGSGPLR AARDSIELRR GACVRTTGEE LCNGHGLWVK
121LTKEQLAEHL GDCGLQEGWL LVCRPAEGGA RLVPIDTPNH LQRQQQLFGV DYRPVLRWEQ
181VVDLTYSHRL GSRPQPAEAY AEAVQRLLYV PPTWTYECDE DLIHFLYDHL GKEDENLGSV
241KQYVESIDVS SYTEEFNVSC LTDSNADTYW ESDGSQCQHW VRLTMKKGTI VKKLLLTVDT
301TDDNFMPKRV VVYGGEGDNL KKLSDVSIDE TLIGDVCVLE DMTVHLPIIE IRIVECRDDG
361IDVRLRGVKI KSSRQRELGL NADLFQPTSL VRYPRLEGTD PEVLYRRAVL LQRFIKILDS
421VLHHLVPAWD HTLGTFSEIK QVKQFLLLSR QRPGLVAQCL RDSESSKPSF MPRLYINRRL
481AMEHRACPSR DPACKNAVFT QVYEGLKPSD KYEKPLDYRW PMRYDQWWEC KFIAEGIIDQ
541GGGFRDSLAD MSEELCPSSA DTPVPLPFFV RTANQGNGTG EARDMYVPNP SCRDFAKYEW
601IGQLMGAALR GKEFLVLALP GFVWKQLSGE EVSWSKDFPA VDSVLVKLLE VMEGMDKETF
661EFKFGKELTF TTVLSDQQVV ELIPGGAGIV VGYGDRSRFI QLVQKARLEE SKEQVAAMQA
721GLLKVVPQAV LDLLTWQELE KKVCGDPEVT VDALRKLTRF EDFEPSDSRV QYFWEALNNF
781TNEDRSRFLR FVTGRSRLPA RIYIYPDKLG YETTDALPES STCSSTLFLP HYASAKVCEE
841KLRYAAYNCV AIDTDMSPWE E
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

861 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.