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GTPBP3 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

GTPBP3 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$504.00each
Catalog Number: LS-G131004-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human GTPBP3 (NM_001195422), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
GTP-binding protein 3 | GTPBG3 | MSS1 | MTGP1 | GTPBP3 | THDF1
Origin Species
Human
NCBI GenBank
NM_032620
SwissProt
Q969Y2

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
54.9 kDa
Protein Type
Lysate
Amino Acid Sequence
1MVHSPTCPHP CFLLVPASEP QFPHLQTPDP GDAVWNVRWA LCTRRSSGAP APGSGATIFA
61LSSGQGRCGI AVIRTSGPAS GHALRILTAP RDLPLARHAS LRLLSDPRSG EPLDRALVLW
121FPGPQSFTGE DCVEFHVHGG PAVVSGVLQA LGSVPGLRPA EAGEFTRRAF ANGKLNLTEV
181EGLADLIHAE TEAQRRQALR QLDGELGHLC RGWAETLTKA LAHVEAYIDF GEDDNLEEGV
241LEQADIEVRA LQVALGAHLR DARRGQRLRS GVHVVVTGPP NAGKSSLVNL LSRKPVSIVS
301PEPGTTRDVL ETPVDLAGFP VLLSDTAGLR EGVGPVEQEG VRRARERLEQ ADLILAMLDA
361SDLASPSSCN FLATVVASVG AQSPSDSSQR LLLVLNKSDL LSPEGPGPGP DLPPHLLLSC
421LTGEGLDGLL EALRKELAAV CGDPSTDPPL LTRARHQHHL QGCLDALGHY KQSKDLALAA
481EALRVARGHL TRLTGGGGTE EILDIIFQDF CVGK
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

514 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.