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GTPBP3 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

GTPBP3 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$725.00each
Catalog Number: LS-G107470-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human GTPBP3 (NM_133644), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
GTP-binding protein 3 | GTPBG3 | MSS1 | MTGP1 | GTPBP3 | THDF1
Origin Species
Human
NCBI GenBank
NM_032620
SwissProt
Q969Y2

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
55.4 kda
Protein Type
Lysate
Amino Acid Sequence
1MWRGLWTLAA QAARGPRRLC TRRSSGAPAP GSGATIFALS SGQGRCGIAV IRTSGPASGH
61ALRILTAPRD LPLARHASLR LLSDPRSGEP LDRALVLWFP GPQSFTGEDC VEFHVHGGPA
121VVSGVLQALG SVPGLRPAEA GEFTRRAFAN GKLNLTEVEG LADLIHAETE AQRRQALRQL
181DGELGHLCRG WAETLTKALA HVEAYIDFGE DDNLEEGVLE QGGSTWWWGR KTPHISPQRL
241PSLSLSACLL SPTADIEVRA LQVALGAHLR DARRGQRLRS GVHVVVTGPP NAGKSSLVNL
301LSRKPVSIVS PEPGTTRDVL ETPVDLAGFP VLLSDTAGLR EGVGPVEQEG VRRARERLEQ
361ADLILAMLDA SDLASPSSCN FLATVVASVG AQSPSDSSQR LLLVLNKSDL LSPEGPGPGP
421DLPPHLLLSC LTGEGLDGLL EALRKELAAV CGDPSTDPPL LTRARHQHHL QGCLDALGHY
481KQSKDLALAA EALRVARGHL TRLTGGGGTE EILDIIFQDF CVGK
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

524 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.