Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
GRIPAP1 / GRASP1 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G102729-100
Catalog Number: LS-G102729
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human GRASP1(GRIPAP1) (NM_207672), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- GRIPAP1 | GRIP1 associated protein 1 | KIAA1167 | GRASP1 | MPMGp800B12492Q3 | GRASP-1 | GRIP1-associated protein 1
- Origin Species
- Human
- NCBI GenBank
- NM_020137
- SwissProt
- Q4V328
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 71.7 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MAQALSEEEF QRMQAQLLEL RTNNYQLSDE LRKNGVELTS LRQKVAYLDK EFSKAQKALS61KSKKAQEVEV LLSENEMLQA KLHSQEEDFR LQNSTLMAEF SKERYGKEAG KFSAVSEGQG121DPPGGPAPTV LAPMPLAEVE LKWEMEKEEK RLLWEQLQGL ESSKQAETSR LQEELAKLSE181KLKKKQESFC RLQTEKETLF NDSRNKIEEL QQRKEADHKA QLARTQKLQQ ELEAANQSLA241ELRDQRQGER LEHAAALRAL QDQVSIQSAD AQEQVEGLLA ENNALRTSLA ALEQIQTAKT301QELNMLREQT TGLAAELQQQ QAEYEDLMGQ KDDLNSQLQE SLRANSRLLE QLQEIGQEKE361QLTQELQEAR KSAEKRKAML DELAMETLQE KSQHKEELGA VRLRHEKEVL GVRARYEREL421RELHEDKKRQ EEELRGQIRE EKARTRELET LQQTVEELQA QVHSMDGAKG WFERRLKEAE481GKEEELQDVR DQLEQAQEER DCHLKTISSL KQEVKDTVDG QRILEKKGSA ALKDLKRQLH541LERKRADKLQ ERLQDILTNS KSRSGLEELV LSEMNSPSRT QTGDSSSISS FSYREILREK601ESSAVPARVR GKEEPTAPAS LNPKITRTRPLEQKLISEEDLAANDILDYKDDDDKV
625 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.