Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
GRIPAP1 / GRASP1 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G101638-100
Catalog Number: LS-G101638
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human GRASP1(GRIPAP1) (NM_020137), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- GRIPAP1 | GRIP1 associated protein 1 | KIAA1167 | GRASP1 | MPMGp800B12492Q3 | GRASP-1 | GRIP1-associated protein 1
- Origin Species
- Human
- NCBI GenBank
- NM_020137
- SwissProt
- Q4V328
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 95.8 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MAQALSEEEF QRMQAQLLEL RTNNYQLSDE LRKNGVELTS LRQKVAYLDK EFSKAQKALS61KSKKAQEVEV LLSENEMLQA KLHSQEEDFR LQNSTLMAEF SKLCSQMEQL EQENQQLKEG121AAGAGVAQAG PLVDGELLRL QAENTALQKN VAALQERYGK EAGKFSAVSE GQGDPPGGPA181PTVLAPMPLA EVELKWEMEK EEKRLLWEQL QGLESSKQAE TSRLQEELAK LSEKLKKKQE241SFCRLQTEKE TLFNDSRNKI EELQQRKEAD HKAQLARTQK LQQELEAANQ SLAELRDQRQ301GERLEHAAAL RALQDQVSIQ SADAQEQVEG LLAENNALRT SLAALEQIQT AKTQELNMLR361EQTTGLAAEL QQQQAEYEDL MGQKDDLNSQ LQESLRANSR LLEQLQEIGQ EKEQLTQELQ421EARKSAEKRK AMLDELAMET LQEKSQHKEE LGAVRLRHEK EVLGVRARYE RELRELHEDK481KRQEEELRGQ IREEKARTRE LETLQQTVEE LQAQVHSMDG AKGWFERRLK EAEESLQQQQ541QEQEEALKQC REQHAAELKG KEEELQDVRD QLEQAQEERD CHLKTISSLK QEVKDTVDGQ601RILEKKGSAA LKDLKRQLHL ERKRADKLQE RLQDILTNSK SRSGLEELVL SEMNSPSRTQ661TGDSSSISSF SYREILREKE SSAVPARSLS SSPQAQPPRP AELSDEEVAE LFQRLAETQQ721EKWMLEEKVK HLEVSSASMA EDLCRKSAII ETYVMDSRID VSVAAGHTDR SGLGSVLRDL781VKPGDENLRE MNKKLQNMLE EQLTKNMHLH KDMEVLSQEI VRLSKECVGP PDPDLEPGET841STRTRPLEQKLISEEDLAANDILDYKDDDDKV
841 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.