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GRHL3 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

GRHL3 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$725.00each
Catalog Number: LS-G110730-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human GRHL3 (NM_021180), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
Grainyhead-like 3 (Drosophila) | GRHL3 | Sister of mammalian grainyhead | Sister-of-mammalian grainyhead | TFCP2L4 | Transcription factor hSOM1 | SOM
Origin Species
Human
NCBI GenBank
NM_021180
SwissProt
Q8TE85

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
68.2 kDa
Protein Type
Lysate
Amino Acid Sequence
1MWMNSILPIF LFRSVRLLKN DPVNLQKFSY TSEDEAWKTY LENPLTAATK AMMRVNGDDD
61SVAALSFLYD YYMGPKEKRI LSSSTGGRND QGKRYYHGME YETDLTPLES PTHLMKFLTE
121NVSGTPEYPD LLKKNNLMSL EGALPTPGKA APLPAGPSKL EAGSVDSYLL PTTDMYDNGS
181LNSLFESIHG VPPTQRWQPD STFKDDPQES MLFPDILKTS PEPPCPEDYP SLKSDFEYTL
241GSPKAIHIKS GESPMAYLNK GQFYPVTLRT PAGGKGLALS SNKVKSVVMV VFDNEKVPVE
301QLRFWKHWHS RQPTAKQRVI DVADCKENFN TVEHIEEVAY NALSFVWNVN EEAKVFIGVN
361CLSTDFSSQK GVKGVPLNLQ IDTYDCGLGT ERLVHRAVCQ IKIFCDKGAE RKMRDDERKQ
421FRRKVKCPDS SNSGVKGCLL SGFRGNETTY LRPETDLETP PVLFIPNVHF SSLQRSGGAA
481PSAGPSSSNR LPLKRTCSPF TEEFEPLPSK QAKEGDLQRV LLYVRRETEE VFDALMLKTP
541DLKGLRNAIS EKYGFPEENI YKVYKKCKRG ILVNMDNNII QHYSNHVAFL LDMGELDGKI
601QIILKEL
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

607 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.