LSBio
GRHL3 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

GRHL3 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all GRHL3 antibodies →
$504.00each
Catalog Number: LS-G103747-100

For bulk orders or custom formulations:

Request a Quote

Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human GRHL3 (NM_198174), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
Grainyhead-like 3 (Drosophila) | GRHL3 | Sister of mammalian grainyhead | Sister-of-mammalian grainyhead | TFCP2L4 | Transcription factor hSOM1 | SOM
Origin Species
Human
NCBI GenBank
NM_021180
SwissProt
Q8TE85

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
56.9 kDa
Protein Type
Lysate
Amino Acid Sequence
1MRVNGDDDSV AALSFLYDYY MGPKEKRILS SSTGGRNDQG KRYYHGMEYE TDLTPLESPT
61HLMKFLTENV SGTPEYPDLL KKNNLMSLEG ALPTPGKAAP LPAGPSKLEA GSVDSYLLPT
121TDMYDNGSLN SLFESIHGVP PTQRWQPDST FKDDPQESML FPDILKTSPE PPCPEDYPSL
181KSDFEYTLGS PKAIHIKSGE SPMAYLNKGQ FYPVTLRTPA GGKGLALSSN KVKSVVMVVF
241DNEKVPVEQL RFWKHWHSRQ PTAKQRVIDV ADCKENFNTV EHIEEVAYNA LSFVWNVNEE
301AKVFIGVNCL STDFSSQKGV KGVPLNLQID TYDCGLGTER LVHRAVCQIK IFCDKGAERK
361MRDDERKQFR RKVKCPDSSN SGVKGCLLSG FRGNETTYLR PETDLETPPV LFIPNVHFSS
421LQRSGGAAPS AGPSSSNRLP LKRTCSPFTE EFEPLPSKQA KEGDLQRVLL YVRRETEEVF
481DALMLKTPDL KGLRNAISEK YGFPEENIYK VYKKCKRGIL VNMDNNIIQH YSNHVAFLLD
541MGELDGKIQI ILKEL
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

555 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.