Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
GPR48 / LGR4 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G111695-100
Catalog Number: LS-G111695
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human GPR48(LGR4) (NM_018490), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- G-protein coupled receptor 48 | G protein-coupled receptor 48 | GPR48 | LGR4
- Origin Species
- Human
- NCBI GenBank
- NM_018490
- SwissProt
- Q9BXB1
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 104.3 kda
- Protein Type
- Lysate
- Amino Acid Sequence
1MPGPLGLLCF LALGLLGSAG PSGAAPPLCA APCSCDGDRR VDCSGKGLTA VPEGLSAFTQ61ALDISMNNIT QLPEDAFKNF PFLEELQLAG NDLSFIHPKA LSGLKELKVL TLQNNQLKTV121PSEAIRGLSA LQSLRLDANH ITSVPEDSFE GLVQLRHLWL DDNSLTEVPV HPLSNLPTLQ181ALTLALNKIS SIPDFAFTNL SSLVVLHLHN NKIRSLSQHC FDGLDNLETL DLNYNNLGEF241PQAIKALPSL KELGFHSNSI SVIPDGAFDG NPLLRTIHLY DNPLSFVGNS AFHNLSDLHS301LVIRGASMVQ QFPNLTGTVH LESLTLTGTK ISSIPNNLCQ EQKMLRTLDL SYNNIRDLPS361FNGCHALEEI SLQRNQIYQI KEGTFQGLIS LRILDLSRNL IHEIHSRAFA TLGPITNLDV421SFNELTSFPT EGLNGLNQLK LVGNFKLKEA LAAKDFVNLR SLSVPYAYQC CAFWGCDSYA481NLNTEDNSLQ DHSVAQEKGT ADAANVTSTL ENEEHSQIII HCTPSTGAFK PCEYLLGSWM541IRLTVWFIFL VALFFNLLVI LTTFASCTSL PSSKLFIGLI SVSNLFMGIY TGILTFLDAV601SWGRFAEFGI WWETGSGCKV AGFLAVFSSE SAIFLLMLAT VERSLSAKDI MKNGKSNHLK661QFRVAALLAF LGATVAGCFP LFHRGEYSAS PLCLPFPTGE TPSLGFTVTL VLLNSLAFLL721MAVIYTKLYC NLEKEDLSEN SQSSMIKHVA WLIFTNCIFF CPVAFFSFAP LITAISISPE781IMKSVTLIFF PLPACLNPVL YVFFNPKFKE DWKLLKRRVT KKSGSVSVSI SSQGGCLEQD841FYYDCGMYSH LQGNLTVCDC CESFLLTKPV SCKHLIKSHS CPALAVASCQ RPEGYWSDCG901TQSAHSDYAD EEDSFVSDSS DQVQACGRAC FYQSRGFPLV RYAYNLPRVK DTRTRPLEQKLISEEDLAANDILDYKDDDDKV
951 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.