Western blot analysis of extracts of various cell lines, using LGR4 antibody at 1:3000 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking. An ECL Kit was used for detection and the exposure time was 1s.

GPR48 / LGR4 Antibody

Primary AntibodyLSBio Guarantee

Antibody:	Rabbit Polyclonal
Applications:	Western Blot
Reactivity:	Human, Mouse, Rat
Format:	Liquid

$281.00each

Catalog Number: LS-C747748-20

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Overview

Description: LGR4 antibody LS-C747748 is an unconjugated rabbit polyclonal antibody to LGR4 (GPR48) from human. It is reactive with human, mouse and rat. Validated for WB.
Application: WB
Reactivity: Hu, Ms, Rt
Intended Use: RUO
Guarantees: This antibody carries the LSBio 100% Guarantee. ✓
Category: Antibodies > Primary Antibodies > Polyclonal Antibodies
Alternative Names: G-protein coupled receptor 48 | G protein-coupled receptor 48 | GPR48 | LGR4
Recommended Dilution: WB: 1:1000 - 1:3000
UniProt Number: Q9BXB1
NCBI GenBank: NM_018490, NP_060960.2
SwissProt: Q9BXB1

Target

Target: Human GPR48 / LGR4
Antigen Species: Hu
Gene Name: LGR4

Antibody

Host Species: Rb
Clonality: Polyclonal
Isotype: IgG
Origin Species: Hu

Immunogen

Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 320-540 of human LGR4 (NP_060960.2). HLESLTLTGTKISSIPNNLCQEQKMLRTLDLSYNNIRDLPSFNGCHALEEISLQRNQIYQIKEGTFQGLISLRILDLSRNLIHEIHSRAFATLGPITNLDVSFNELTSFPTEGLNGLNQLKLVGNFKLKEALAAKDFVNLRSLSVPYAYQCCAFWGCDSYANLNTEDNSLQDHSVAQEKGTADAANVTSTLENEEHSQIIIHCTPSTGAFKPCEYLLGSWM
Immunogen Type: Fusion-P