Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
GLUP / PACRG Protein (Over-Expression Lysate Myc-DDK (Flag))
ProteinLSBio Guarantee
| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G106301-100
Catalog Number: LS-G106301
For bulk orders or custom formulations:
Request a QuoteOverview
- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human PACRG (NM_152410), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- GLUP | HAK005771 | PACRG | PARK2 co-regulated | PARK2CRG | RP3-495O10.2 | PARK2 coregulated gene protein
- Origin Species
- Human
- SwissProt
- Q96M98
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 33.2 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MVAEKETLSL NKCPDKMPKR TKLLAQQPLP VHQPHSLVSE GFTVKAMMKN SVVRGPPAAG61AFKERPTKPT AFRKFYERGD FPIALEHDSK GNRIAWKVEI EKLDYHHYLP LFFDGLCEMT121FPYEFFARQG IHDMLEHGGN KILPVLPQLI IPIKNALNLR NRQVICVTLK VLQHLVVSAE181MVGKALVPYY RQILPVLNIF KNMNGSYSLP RLECSGAIMA RCNLDHLGSS DPPTSASQVA241EIIVNSGDGI DYSQQKRENI GDLIQETLEA FERYGGENAF INIKYVVPTY ESCLLNTRTRPLEQKLISEEDLAANDILDYKDDDDKV
296 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.