Anti-PACRG antibody IHC of human colon. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

PathPlus™ GLUP / PACRG Antibody

Primary AntibodyLSBio Guarantee

Antibody:	Rabbit Polyclonal
Applications:	Enzyme-Linked Immunosorbent Assay, Immunohistochemistry (general), Immunohistochemistry, Paraffin, Western Blot
Reactivity:	Human
Format:	Liquid

$526.00each

Catalog Number: LS-B127-50

Unit Size

Quantity

For bulk orders or custom formulations:

Overview

Description: PACRG antibody LS-B127 is an unconjugated rabbit polyclonal antibody to PACRG (GLUP) from human. It is reactive with human, mouse and chicken. Validated for ELISA, IHC and WB. Cited in 1 publication.
Application: ELISA, IHC, IHC-P, WB
Reactivity: Hu
Predicted Reactivity: Chicken, Mouse, Zebrafish
Intended Use: Research Use Only
Guarantees: This product carries the LSBio 100% Guarantee. ✓
Category: Antibodies > Primary Antibodies > Polyclonal Antibodies
Alternative Names: GLUP | HAK005771 | PACRG | PARK2 co-regulated | PARK2CRG | RP3-495O10.2 | PARK2 coregulated gene protein
UniProt Number: Q96M98
NCBI GenBank: NP_689623.2
SwissProt: Q96M98

Recommended Dilution

Application	Dilution
ELISA	1:2000 - 1:10000
IHC-P	2.5 µg/ml
WB	1:500 - 1:3000

Target

Target: GLUP / PACRG
Antigen Species: Human
Specificity: A BLAST analysis was used to suggest reactivity with this protein from human, mouse and chicken based on 100% homology for the immunogen sequence. Cross reactivity with PACRG protein from zebrafish and rat may occur as this sequence only varies by one amino acid residue (94% homology). Expect reactivity with both splice variants of this protein. Cross reactivity with PACRG homologues from other sources has not been determined.
Epitope: A BLAST analysis was used to suggest reactivity with this protein from human, mouse and chicken based on 100% homology for the immunogen sequence. Cross reactivity with PACRG protein from zebrafish and rat may occur as this sequence only varies by one amino acid residue (94% homology). Expect reactivity with both splice variants of this protein. Cross reactivity with PACRG homologues from other sources has not been determined.
Gene Name: PACRG

Antibody

Host Species: Rabbit
Clonality: Polyclonal
Origin Species: Human

Immunogen

Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region of Human PACRG protein.
Immunogen Type: Synthetic Peptide (Linear)

Format

Conjugate/Label: Unconjugated
Format: Liquid
Formulation: 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Concentration: 1 mg/mL
Animal Free: No
Carrier-Free: Yes
Preservative: Yes
Purification Method: Affinity Purified

Storage & Handling

Storage Conditions: Store vial at -20°C prior to opening. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20°C or below. Avoid freeze-thaw cycles.
Recommended Storage Buffer: 0.02 M Potassium Phosphate, 0.15 M NaCl

PARK2 and PACRG are commonly downregulated in clear-cell renal cell carcinoma and are associated with aggressive disease and poor clinical outcome. Toma MI, Wuttig D, Kaiser S, Herr A, Weber T, Zastrow S, Koch R, Meinhardt M, Baretton GB, Wirth MP, Fuessel S. Genes, chromosomes & cancer. 2013 Mar;52:265-73. PubMed: 23125027

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