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GLIS3 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

GLIS3 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$725.00each
Catalog Number: LS-G119496-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human GLIS3 (NM_001042413), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
GLIS family zinc finger 3 | OK/KNS-cl.4 | Zinc finger protein 515 | Zinc finger protein GLIS3 | GLI-similar 3 | GLIS3 | ZNF515
Origin Species
Human
NCBI GenBank
NM_152629
SwissProt
Q8NEA6

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
99.4 kDa
Protein Type
Lysate
Amino Acid Sequence
1MNGRSCSMSL HRTSGTPQGP RMVSGHHIPA IRAHSGTPGP SPCGSTSSPT MASLANNLHL
61KMPSGGGMAP QNNVAESRIH LPALSPRRQM LTNGKPRFQV TQAGGMSGSH TLKPKQQEFG
121SPFPPNPGKG ALGFGPQCKS IGKGSCNNLV VTSSPMMVQR LGLISPPASQ VSTACNQISP
181SLQRAMNAAN LNIPPSDTRS LISRESLAST TLSLTESQSA SSMKQEWSQG YRALPSLSNH
241GSQNGLDLGD LLSLPPGTSM SSNSVSNSLP SYLFGTESSH SPYPSPRHSS TRSHSARSKK
301RALSLSPLSD GIGIDFNTII RTSPTSLVAY INGSRASPAN LSPQPEVYGH FLGVRGSCIP
361QPRPVPGSQK GVLVAPGGLA LPAYGEDGAL EHERMQQLEH GGLQPGLVNH MVVQHGLPGP
421DSQSAGLFKT ERLEEFPGST VDLPPAPPLP PLPPPPGPPP PYHAHAHLHH PELGPHAQQL
481ALPQATLDDD GEMDGIGGKH CCRWIDCSAL YDQQEELVRH IEKVHIDQRK GEDFTCFWAG
541CPRRYKPFNA RYKLLIHMRV HSGEKPNKCT FEGCEKAFSR LENLKIHLRS HTGEKPYLCQ
601HPGCQKAFSN SSDRAKHQRT HLDTKPYACQ IPGCTKRYTD PSSLRKHVKA HSSKEQQARK
661KLRSSTELHP DLLTDCLTVQ SLQPATSPRD AAAEGTVGRS PGPGPDLYSA PIFSSNYSSR
721SGTAAGAVPP PHPVSHPSPG HNVQGSPHNP SSQLPPLTAV DAGAERFAPS APSPHHISPR
781RVPAPSSILQ RTQPPYTQQP SGSHLKSYQP ETNSSFQPNG IHVHGFYGQL QKFCPPHYPD
841SQRIVPPVSS CSVVPSFEDC LVPTSMGQAS FDVFHRAFST HSGITVYDLP SSSSSLFGES
901LRSGAEDATF LQISTVDRCP SQLSSVYTEG
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

930 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.