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GLIS3 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

GLIS3 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$504.00each
Catalog Number: LS-G106159-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human GLIS3 (NM_152629), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
GLIS family zinc finger 3 | OK/KNS-cl.4 | Zinc finger protein 515 | Zinc finger protein GLIS3 | GLI-similar 3 | GLIS3 | ZNF515
Origin Species
Human
NCBI GenBank
NM_152629
SwissProt
Q8NEA6

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
83.5 kDa
Protein Type
Lysate
Amino Acid Sequence
1MMVQRLGLIS PPASQVSTAC NQISPSLQRA MNAANLNIPP SDTRSLISRE SLASTTLSLT
61ESQSASSMKQ EWSQGYRALP SLSNHGSQNG LDLGDLLSLP PGTSMSSNSV SNSLPSYLFG
121TESSHSPYPS PRHSSTRSHS ARSKKRALSL SPLSDGIGID FNTIIRTSPT SLVAYINGSR
181ASPANLSPQP EVYGHFLGVR GSCIPQPRPV PGSQKGVLVA PGGLALPAYG EDGALEHERM
241QQLEHGGLQP GLVNHMVVQH GLPGPDSQSA GLFKTERLEE FPGSTVDLPP APPLPPLPPP
301PGPPPPYHAH AHLHHPELGP HAQQLALPQA TLDDDGEMDG IGGKHCCRWI DCSALYDQQE
361ELVRHIEKVH IDQRKGEDFT CFWAGCPRRY KPFNARYKLL IHMRVHSGEK PNKCTFEGCE
421KAFSRLENLK IHLRSHTGEK PYLCQHPGCQ KAFSNSSDRA KHQRTHLDTK PYACQIPGCT
481KRYTDPSSLR KHVKAHSSKE QQARKKLRSS TELHPDLLTD CLTVQSLQPA TSPRDAAAEG
541TVGRSPGPGP DLYSAPIFSS NYSSRSGTAA GAVPPPHPVS HPSPGHNVQG SPHNPSSQLP
601PLTAVDAGAE RFAPSAPSPH HISPRRVPAP SSILQRTQPP YTQQPSGSHL KSYQPETNSS
661FQPNGIHVHG FYGQLQKFCP PHYPDSQRIV PPVSSCSVVP SFEDCLVPTS MGQASFDVFH
721RAFSTHSGIT VYDLPSSSSS LFGESLRSGA EDATFLQIST VDRCPSQLSS VYTEG
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

775 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.