Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
GHSR / Ghrelin Receptor Protein (Over-Expression Lysate Myc-DDK (Flag))
ProteinLSBio Guarantee
| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G116855-100
Catalog Number: LS-G116855
For bulk orders or custom formulations:
Request a QuoteOverview
- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human Ghrelin Receptor(GHSR) (NM_004122), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- GHS receptor | GHSR | GH secretagogue receptor | GHRP | GHS-R | GH-releasing peptide receptor | Ghrelin receptor | Ghrp receptor
- Origin Species
- Human
- NCBI GenBank
- NM_198407
- SwissProt
- Q92847
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 32 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MWNATPSEEP GFNLTLADLD WDASPGNDSL GDELLQLFPA PLLAGVTATC VALFVVGIAG61NLLTMLVVSR FRELRTTTNL YLSSMAFSDL LIFLCMPLDL VRLWQYRPWN FGDLLCKLFQ121FVSESCTYAT VLTITALGVE RYFAICFPLR AKVVVTKGRV KLVIFVIWAV AFCSAGPIFV181LVGVEHENGT DPWDTNECRP TEFAVRSGLL TVMVWVSSIF FFLPVFCLTV LYSLIGRKLW241RRRRGDAVVG ASLRDQNHKQ TVKMLGGSQR ALRLSLAGPI LSLCLLPSLTRTRPLEQKLISEEDLAANDILDYKDDDDKV
289 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.