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FOXP4 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

FOXP4 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$504.00each
Catalog Number: LS-G121391-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human FOXP4 (NM_001012426), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
Forkhead box protein P4 | FOXP4 | HFKHLA | Forkhead box P4 | Winged-helix repressor FOXP4 | FKHLA
Origin Species
Human
SwissProt
Q8IVH2

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
73.3 kDa
Protein Type
Lysate
Amino Acid Sequence
1MMVESASETI RSAPSGQNGV GSLSGQADGS SGGATGTTAS GTGREVTTGA DSNGEMSPAE
61LLHFQQQQAL QVARQFLLQQ ASGLSSPGNN DSKQSASAVQ VPVSVAMMSP QMLTPQQMQQ
121ILSPPQLQAL LQQQQALMLQ QEYYKKQQEQ LHLQLLTQQQ AGKPQPKEAL GNKQLAFQQQ
181LLQMQQLQQQ HLLNLQRQGL VSLQPNQASG PLQTLPQAAV CPTDLPQLWK GEGAPGQPAE
241DSVKQEGLDL TGTAATATSF AAPPKVSPPL SHHTLPNGQP TVLTSRRDSS SHEETPGSHP
301LYGHGECKWP GCETLCEDLG QFIKHLNTEH ALDDRSTAQC RVQMQVVQQL EIQLAKESER
361LQAMMAHLHM RPSEPKPFSQ PLNPVPGSSS FSKVTVSAAD SFPDGLVHPP TSAAAPVTPL
421RPPGLGSASL HGGGPARRRS SDKFCSPISS ELAQNHEFYK NADVRPPFTY ASLIRQAILE
481TPDRQLTLNE IYNWFTRMFA YFRRNTATWK NAVRHNLSLH KCFVRVENVK GAVWTVDERE
541YQKRRPPKMT GSPTLVKNMI SGLSYGALNA SYQAALAESS FPLLNSPGML NPGSASSLLP
601LSHDDVGAPV EPLPSNGSSS PPRLSPPQYS HQVQVKEEPA EAEEDRQPGP PLGAPNPSAS
661GPPEDRDLEE ELPGEELS
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

678 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.