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FOXP4 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

FOXP4 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$504.00each
Catalog Number: LS-G107317-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human FOXP4 (NM_138457), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
Forkhead box protein P4 | FOXP4 | HFKHLA | Forkhead box P4 | Winged-helix repressor FOXP4 | FKHLA
Origin Species
Human
SwissProt
Q8IVH2

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
72 kDa
Protein Type
Lysate
Amino Acid Sequence
1MMVESASETI RSAPSGQNGV GSLSGQADGS SGGATGTTAS GTGREVTTGA DSNGEMSPAE
61LLHFQQQQAL QVARQFLLQQ ASGLSSPGNN DSKQSASAVQ VPVSVAMMSP QMLTPQQMQQ
121ILSPPQLQAL LQQQQALMLQ QLQEYYKKQQ EQLHLQLLTQ QQAGKPQPKE ALGNKQLAFQ
181QQLLQMQQLQ QQHLLNLQRQ GLVSLQPNQA SGPLQTLPQA VCPTDLPQLW KGEGAPGQPA
241EDSVKQEGLD LTGTAATATS FAAPPKVSPP LSHHTLPNGQ PTVLTSRRDS SSHEETPGSH
301PLYGHGECKW PGCETLCEDL GQFIKHLNTE HALDDRSTAQ CRVQMQVVQQ LEIQLAKESE
361RLQAMMAHLH MRPSEPKPFS QPVTVSAADS FPDGLVHPPT SAAAPVTPLR PPGLGSASLH
421GGGPARRRSS DKFCSPISSE LAQNHEFYKN ADVRPPFTYA SLIRQAILET PDRQLTLNEI
481YNWFTRMFAY FRRNTATWKN AVRHNLSLHK CFVRVENVKG AVWTVDEREY QKRRPPKMTG
541SPTLVKNMIS GLSYGALNAS YQAALAESSF PLLNSPGMLN PGSASSLLPL SHDDVGAPVE
601PLPSNGSSSP PRLSPPQYSH QVQVKEEPAE AEEDRQPGPP LGAPNPSASG PPEDRDLEEE
661LPGEELS
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

667 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.