Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
FKBP38 / FKBP8 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G114592-100
Catalog Number: LS-G114592
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human FKBP38(FKBP8) (NM_012181), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- 38 kDa FKBP | 38 kDa FK506-binding protein | FKBP-8 | FK506-binding protein 8 | FKBP38 | FKBP8 | HFKBP38 | FKBP-38 | Rotamase | FK506 binding protein 8 | 38kDa | FK506-binding protein 8 (38kD) | FKBPr38 | PPIase FKBP8
- Origin Species
- Human
- NCBI GenBank
- NM_012181
- SwissProt
- Q14318
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 44.5 kda
- Protein Type
- Lysate
- Amino Acid Sequence
1MASCAEPSEP SAPLPAGVPP LEDFEVLDGV EDAEGEEEEE EEEEEEDDLS ELPPLEDMGQ61PPAEEAEQPG ALAREFLAAM EPEPAPAPAP EEWLDILGNG LLRKKTLVPG PPGSSRPVKG121QVVTVHLQTS LENGTRVQEE PELVFTLGDC DVIQALDLSV PLMDVGETAM VTADSKYCYG181PQGSRSPYIP PHAALCLEVT LKTAVDGPDL EMLTGQERVA LANRKRECGN AHYQRADFVL241AANSYDLAIK AITSSAKVDM TFEEEAQLLQ LKVKCLNNLA ASQLKLDHYR AALRSCSLVL301EHQPDNIKAL FRKGKVLAQQ GEYSEAIPIL RAALKLEPSN KTIHAELSKL VKKHAAQRST361ETALYRKMLG NPSRLPAKCP GKGAWSIPWK WLFGATAVAL GGVALSVVIA ARNTRTRPLEQKLISEEDLAANDILDYKDDDDKV
413 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.