Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
FAP-1 / PTPN13 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G115424-100
Catalog Number: LS-G115424
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human PTPN13 (NM_006264), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- FAP-1 | HPTP1E | HPTPE1 | Fas associated phosphatase 1 | Phosphatase rip | PTP-BAS | PTP-E1 | PTP-BL | PTP1E | PTPLE | PNP1 | PTPL1 | PTPN13
- Origin Species
- Human
- NCBI GenBank
- NM_006264
- SwissProt
- Q12923
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 254.51 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MHVSLAEALE VRGGPLQEEE IWAVLNQSAE SLQELFRKVS LADPAALGFI ISPWSLLLLP61SGSVSFTDEN ISNQDLRAFT APEVLQNQSL TSLSDVEKIH IYSLGMTLYW GADYEVPQSQ121PIKLGDHLNS ILLGMCEDVI YARVSVRTVL DACSAHIRNS NCAPSFSYVK HLVKLVLGNL181SGTDQLSCNS EQKPDRSQAI RDRLRGKGLP TEPVRRYKTY HGDVFSTSSE SPSIISSESD241FRQVRRSEAS KRFESSSGLP GVDETLSQGQ SQRPSRQYET PFEGNLINQE IMLKRQEEEL301MQLQAKMALR QSRLSLYPGD TIKASMLDIT RDPLREIALE TAMTQRKLRN FFGPEFVKMT361IEPFISLDLP RSILTKKGKN EDNRRKVNIM LLNGQRLELT CDTKTICKDV FDMVVAHIGL421VEHHLFALAT LKDNEYFFVD PDLKLTKVAP EGWKEEPKKK TKATVNFTLF FRIKFFMDDV481SLIQHTLTCH QYYLQLRKDI LEERMHCDDE TSLLLASLAL QAEYGDYQPE VHGVSYFRME541HYLPARVMEK LDLSYIKEEL PKLHNTYVGA SEKETELEFL KVCQRLTEYG VHFHRVHPEK601KSQTGILLGV CSKGVLVFEV HNGVRTLVLR FPWRETKKIS FSKKKITLQN TSDGIKHGFQ661TDNSKICQYL LHLCSYQHKF QLQMRARQSN QDAQDIERAS FRSLNLQAES VRGFNMGRAI721STGSLASSTL NKLAVRPLSV QAEILKRLSC SELSLYQPLQ NSSKEKNDKA SWEEKPREMS781KSYHDLSQAS LYPHRKNVIV NMEPPPQTVA ELVGKPSHQM SRSDAESLAG VTKLNNSKSV841ASLNRSPERR KHESDSSSIE DPGQAYVLDV LHKRWSIVSS PEREITLVNL KKDAKYGLGF901QIIGGEKMGR LDLGIFISSV APGGPADLDG CLKPGDRLIS VNSVSLEGVS HHAAIEILQN961APEDVTLVIS QPKEKISKVP STPVHLTNEM KNYMKKSSYM QDSAIDSSSK DHHWSRGTLR1021HISENSFGPS GGLREGSLSS QDSRTESASL SQSQVNGFFA SHLGDQTWQE SQHGSPSPSV1081ISKATEKETF TDSNQSKTKK PGISDVTDYS DRGDSDMDEA TYSSSQDHQT PKQESSSSVN1141TSNKMNFKTF SSSPPKPGDI FEVELAKNDN SLGISVTVLF DKGGVNTSVR HGGIYVKAVI1201PQGAAESDGR IHKGDRVLAV NGVSLEGATH KQAVETLRNT GQVVHLLLEK GQSPTSKEHV1261PVTPQCTLSD QNAQGQGPEK VKKTTQVKDY SFVTEENTFE VKLFKNSSGL GFSFSREDNL1321IPEQINASIV RVKKLFPGQP AAESGKIDVG DVILKVNGAS LKGLSQQEVI SALRGTAPEV1381FLLLCRPPPG VLPEIDTALL TPLQSPAQVL PNSSKDSSQP SCVEQSTSSD ENEMSDKSKK1441QCKSPSRRDS YSDSSGSGED DLVTAPANIS NSTWSSALHQ TLSNMVSQAQ SHHEAPKSQE1501DTICTMFYYP QKIPNKPEFE DSNPSPLPPD MAPGQSYQPQ SESASSSSMD KYHIHHISEP1561TRQENWTPLK NDLENHLEDF ELEVELLITL IKSEKGSLGF TVTKGNQRIG CYVHDVIQDP1621AKSDGRLKPG DRLIKVNDTD VTNMTHTDAV NLLRAASKTV RLVIGRVLEL PRIPMLPHLL1681PDITLTCNKE ELGFSLCGGH DSLYQVVYIS DINPRSVAAI EGNLQLLDVI HYVNGVSTQG1741MTLEEVNRAL DMSLPSLVLK ATRNDLPVVP SSKRSAVSAP KSTKGNGSYS VGSCSQPALT1801PNDSFSTVAG EEINEISYPK GKCSTYQIKG SPNLTLPKES YIQEDDIYDD SQEAEVIQSL1861LDVVDEEAQN LLNENNAAGY SCGPGTLKMN GKLSEERTED TDCDGSPLPE YFTEATKMNG1921CEEYCEEKVK SESLIQKPQE KKTDDDEITW GNDELPIERT NHEDSDKDHS FLTNDELAVL1981PVVKVLPSGK YTGANLKSVI RVLRGLLDQG IPSKELENLQ ELKPLDQCLI GQTKENRRKN2041RYKNILPYDA TRVPLGDEGG YINASFIKIP VGKEEFVYIA CQGPLPTTVG DFWQMIWEQK2101STVIAMMTQE VEGEKIKCQR YWPNILGKTT MVSNRLRLAL VRMQQLKGFV VRAMTLEDIQ2161TREVRHISHL NFTAWPDHDT PSQPDDLLTF ISYMRHIHRS GPIITHCSAG IGRSGTLICI2221DVVLGLISQD LDFDISDLVR CMRLQRHGMV QTEDQYIFCY QVILYVLTRL QAEEEQKQQP2281QLLKTRTRPLEQKLISEEDLAANDILDYKDDDDKV
2284 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.