Western blot of FAP-1 using Polyclonal Antibody to FAP-1 at 1:2000. In ovarian carcinoma cell lines FAP-1 expression was detected in SK-OV-3 and OVCAR-3, but not in BG-1 or HEY. Human Jurkat T and 293 kidney cell lines were used as negative and positive controls, respectively.
FAP-1 / PTPN13 Antibody (aa1279-1883)
Primary AntibodyLSBio Guarantee
| Antibody: | Rabbit Polyclonal |
| Applications: | Immunohistochemistry (general), Immunohistochemistry, Frozen, Immunohistochemistry, Paraffin, Immunoprecipitation, Western Blot |
| Reactivity: | Human |
| Format: | Liquid |
$394.00each
Catalog Number: LS-C148268-50
Catalog Number: LS-C148268
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- Description
- PTPN13 antibody LS-C148268 is an unconjugated rabbit polyclonal antibody to human PTPN13 (FAP-1) (aa1279-1883). Validated for IHC, IP and WB. Cited in 1 publication.
- Application
- IHC, IHC-F, IHC-P, IP, WB
- Reactivity
- Hu
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Alternative Names
- FAP-1 | HPTP1E | HPTPE1 | Fas associated phosphatase 1 | Phosphatase rip | PTP-BAS | PTP-E1 | PTP-BL | PTP1E | PTPLE | PNP1 | PTPL1 | PTPN13
- Recommended Dilution: IHC-P
- 1:1000 - 1:5000
- Recommended Dilution: IP
- 1:50 - 1:200
- Recommended Dilution: WB
- 1:1000 - 1:2000
- UniProt Number
- Q12923
- NCBI GenBank
- NM_006264 | NP_006255.1
- SwissProt
- Q12923
Target
- Target
- Human FAP-1 / PTPN13
- Antigen Species
- Human
- Epitope
- FAP-1 typically migrates at ~270 kD on western blots. Thus for SDS-PAGE and western blot, the researcher should use techniques optimized for the transfer of proteins with high molecular weights. The actual molecular weight of FAP-1 observed depends on the isoform expression.
- Gene Name
- PTPN13
Antibody
- Host Species
- Rabbit
- Clonality
- Polyclonal
Immunogen
- Immunogen
- Recombinant protein corresponding to aa 1279-1883 of human FAP-1 protein, GenBank no. NP_542414.1. This peptide sequence is conserved in multiple isoforms of FAP-1. These FAP-1 isoforms are of varying amino acid (aa) lengths and include: (1) NP_542414.1 = 2485 aa, FAP-1 isoform 1. (2) BAD92141.1 = 2434 aa, FAP-1 isoform 2. (3) NP_542415.1 = 2294 aa, FAP-1 isoform 3. (4) NP_542416.1 = 2490 aa, FAP-1 isoform 4. (5) AAH39610.1 = 604 aa, FAP-1. (6) AAF63474.1 =499 aa, FAP-1.
- Immunogen Type
- Recombinant Protein Fragment
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- Antiserum, 0.05% sodium azide
- Volume
- 50 Microliter
- Preservative
- Yes
- Purification Method
- Unpurified (Serum / Ascites / Supernatant)
Storage & Handling
- Storage Conditions
- Store at -20°C. Aliquot to avoid freeze/thaw cycles.
The tyrosine phosphatase PTPN13/FAP-1 links calpain-2, TBI and tau tyrosine phosphorylation. Wang Y, Hall RA, Lee M, Kamgar-Parsi A, Bi X, Baudry M. Scientific reports. 2017;7:11771. PubMed: 28924170 PMC: PMC5603515