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FAM83D Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

FAM83D Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all FAM83D antibodies →
$725.00each
Catalog Number: LS-G109354-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human FAM83D (NM_030919), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
DJ616B8.3 | FAM83D | Protein FAM83D | Spindle protein CHICA | C20orf129 | CHICA
Origin Species
Human
NCBI GenBank
NM_030919

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
67.5 kDa
Protein Type
Lysate
Amino Acid Sequence
1MARACLIQRL PIKRDCTPVF VRGLSSPSAA MALLSEGLDE VPAACLSPCG PPNPTELFSE
61SRRLALEELV AGGPEAFAAF LRRERLARFL NPDEVHAILR AAERPGEEGA AAAAAAEDSF
121GSSHDCSSGT YFPEQSDLEP PLLELGWPAF YQGAYRGATR VETHFQPRGA GEGGPYGCKD
181ALRQQLRSAR EVIAVVMDVF TDIDIFRDLQ EICRKQGVAV YILLDQALLS QFLDMCMDLK
241VHPEQEKLMT VRTITGNIYY ARSGTKIIGK VHEKFTLIDG IRVATGSYSF TWTDGKLNSS
301NLVILSGQVV EHFDLEFRIL YAQSKPISPK LLSHFQSSNK FDHLTNRKPQ SKELTLGNLL
361RMRLARLSST PRKADLDPEM PAEGKAERKP HDCESSTVSE EDYFSSHRDE LQSRKAIDAA
421TQTEPGEEMP GLSVSEVGTQ TSITTACAGT QTAVITRIAS SQTTIWSRST TTQTDMDENI
481LFPRGTQSTE GSPVSKMSVS RSSSLKSSSS VSSQGSVASS TGSPASIRTT DFHNPGYPKY
541LGTPHLELYL SDSLRNLNKE RQFHFAGIRS RLNHMLAMLS RRTLFTENHL GLHSGNFSRV
601NLLAVRDVAL YPSYQ
SGPTRTRRLEQKLISEEDLAANDILDYKDDDDKV

615 aa + 34 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.