Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
FAM117B / ALS2CR13 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G129053-100
Catalog Number: LS-G129053
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human ALS2CR13(FAM117B) (NM_173511), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- ALS2CR13 | FAM117B | Protein FAM117B
- Origin Species
- Human
- NCBI GenBank
- NM_173511
- SwissProt
- Q6P1L5
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 61.8 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MSQRVRRNGS PTPAGSLGGG AVATAGGPGS RLQPMRATVP FQLKQQQQQQ HGSPTRSGGG61GGGNNNGGCC GGASGPAGGG GGGGPRTASR STSPTRGGGN AAARTSPTVA TQTGASATST121RGTSPTRSAA PGARGSPPRP PPPPPLLGTV SSPSSSPTHL WTGEVSAAPP PARVRHRRRS181PEQSRSSPEK RSPSAPVCKA GDKTRQPSSS PSSIIRRTSS LDTLAAPYLA GHWPRDSHGQ241AAPCMRDKAT QTESAWAEEY SEKKKGSHKR SASWGSTDQL KEIAKLRQQL QRSKHSSRHH301RDKERQSPFH GNHAAINQCQ APVPKSALIP VIPITKSTGS RFRNSVEGLN QEIEIIIKET361GEKEEQLIPQ DIPDGHRAPP PLVQRSSSTR SIDTQTPGGA DRGSNNSSRS QSVSPTSFLT421ISNEGSEESP CSADDLLVDP RDKENGNNSP LPKYATSPKP NNSYMFKREP PEGCERVKVF481EECSPKQLHE IPAFYCPDKN KVNFIPKSGS AFCLVSILKP LLPTPDLTLK GSGHSLTVTT541GMTTTLLQPI AVASLSTNTE QDRVSRGTST VMPSASLLPP PEPIEEAEGTRTRPLEQKLISEEDLAANDILDYKDDDDKV
589 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.