Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
ESRP2 / RBM35B Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G109660-100
Catalog Number: LS-G109660
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human ESRP2(ESRP2) (NM_024939), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- ESRP2 | RBM35B | RNA binding motif protein 35A | RNA-binding protein 35B | RNA-binding motif protein 35B | RNA binding motif protein 35B
- Origin Species
- Human
- NCBI GenBank
- NM_024939
- SwissProt
- Q9H6T0
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 77.2 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MTPPPPPPPP PGPDPAADPA ADPCPWPGSL VVLFGATAGA LGRDLGSDET DLILLVWQVV61EPRSRQVGTL HKSLVRAEAA ALSTQCREAS GLSADSLARA EPLDKVLQQF SQLVNGDVAL121LGGGPYMLCT DGQQLLRQVL HPEASRKNLV LPDMFFSFYD LRREFHMQHP STCPARDLTV181ATMAQGLGLE TDATEDDFGV WEVKTMVAVI LHLLKEPSSQ LFSKPEVIKQ KYETGPCSKA241DVVDSETVVR ARGLPWQSSD QDVARFFKGL NVARGGVALC LNAQGRRNGE ALIRFVDSEQ301RDLALQRHKH HMGVRYIEVY KATGEEFVKI AGGTSLEVAR FLSREDQVIL RLRGLPFSAG361PTDVLGFLGP ECPVTGGTEG LLFVRHPDGR PTGDAFALFA CEELAQAALR RHKGMLGKRY421IELFRSTAAE VQQVLNRYAS GPLLPTLTAP LLPIPFPLAP GTGRDCVRLR GLPYTATIED481ILSFLGEAAA DIRPHGVHMV LNQQGRPSGD AFIQMTSAER ALAAAQRCHK KVMKERYVEV541VPCSTEEMSR VLMGGTLGRS GMSPPPCKLP CLSPPTYTTF QATPTLIPTE TAALYPSSAL601LPAARVPAAP TPVAYYPGPA TQLYLNYTAY YPSPPVSPTT VGYLTTPTAA LASAPTSVLS661QSGALVRMQG VPYTAGMKDL LSVFQAYQLP ADDYTSLMPV GDPPRTVLQA PKEWVCLTRTRPLEQKLISEEDLAANDILDYKDDDDKV
717 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.