LSBio
Monoclonal Mouse anti-Mouse ESRP2 / RBM35B Antibody (clone 22C5.H7.A6, WB)

Anti-ESRP2 by western blot shows detection of ESRP2 in transfected 293T cell extracts (lane 2). Lanes ug and 1 contain 5 ug GFP-transfected- and ESRP1-transfected 293T cell lysates, respectively. Briefly, each lane contains approximately 5 ug of lysate. Primary antibody was used at a 1:1000 dilution (PBS-T plus milk) and reacted for O/N at 4C. The membrane was washed and reacted with a 1:10000 dilution of an anti-mouse ECL antibody for 1hr at room temperature. The bands shown are full length FLAG-ESRP2 (~80kDa) and a slightly lower band that is specific to ESRP2. Molecular weight estimation was made by comparison to prestained MW markers.

Monoclonal Mouse anti-Mouse ESRP2 / RBM35B Antibody (clone 22C5.H7.A6, WB)

Primary AntibodyLSBio Guarantee
Antibody:Mouse Monoclonal
Applications:Enzyme-Linked Immunosorbent Assay, Western Blot
Reactivity:Mouse
Format:Liquid
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$586.00each
Catalog Number: LS-C153834-100

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Overview

Description
RBM35B antibody LS-C153834 is an unconjugated mouse monoclonal antibody to mouse RBM35B (ESRP2). Validated for ELISA and WB. Cited in 1 publication.
Application
ELISA, WB
Reactivity
Ms
Intended Use
Research Use Only
Guarantees
This antibody carries the LSBio 100% Guarantee. ✓
Category
Antibodies > Primary Antibodies > Monoclonal Antibodies
Alternative Names
ESRP2 | RBM35B | RNA binding motif protein 35A | RNA-binding protein 35B | RNA-binding motif protein 35B | RNA binding motif protein 35B
Recommended Dilution: ELISA
1:100000
Recommended Dilution: WB
1:1000
UniProt Number
Q9H6T0
NCBI GenBank
NM_024939 | NP_079215.2
SwissProt
Q9H6T0

Target

Target
Mouse ESRP2 / RBM35B
Antigen Species
Mouse
Epitope
This antibody reacts with mouse Esrp-2 protein. A BLAST analysis was used to suggest cross-reactivity with Esrp-2 from mouse based on a 100% homology with the immunizing sequence. Cross-reactivity with Esrp-2 from other sources has not been determined.
Gene Name
ESRP2

Antibody

Host Species
Mouse
Clonality
Monoclonal
Clone
22C5.H7.A6
Isotype
IgG2a
Light Chain
Kappa Light Chain

Immunogen

Immunogen
Anti-Esrp-2 is produced by repeated immunizations of full length recombinant mouse Esrp-2 fusion protein.
Immunogen Type
Fusion Protein (e.g., GST, MBP tag)

Format

Conjugate/Label
Unconjugated
Format
Liquid
Formulation
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Concentration
1 mg/mL
Preservative
Yes
Purification Method
Protein A Affinity

Storage & Handling

Storage Conditions
Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.
Recommended Storage Buffer
0.02 M Potassium Phosphate|0.15 M NaCl

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