TIA-1 Antibody (clone TIA-1)
Catalog Number: LS-C312188-0.1
0.1 mL / $355.00
Antibody: Mouse Monoclonal
Applications: Immunohistochemistry, Frozen, Immunohistochemistry, Paraffin
Reactivity: Human
Format: Liquid
Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
Epsin 1 / EPN1 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G124991-100
Catalog Number: LS-G124991
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human Epsin 1(EPN1) (NM_001130071), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- Epsin-1 | Epsin 1 | EPS-15-interacting protein 1 | EPN1
- Origin Species
- Human
- NCBI GenBank
- NM_013333
- SwissProt
- Q9Y6I3
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 68.9 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MGDQSWLWNQ AAPGVRSPVF ACSVEKGNVP LVLSEHLAHS RDPGSGAVRF LISPEPWASA61ILGTSGLLAS PVLPAALDAV TCQHLPQPSS GSRPISPRIG ALCPLLLQPG TMSTSSLRRQ121MKNIVHNYSE AEIKVREATS NDPWGPSSSL MSEIADLTYN VVAFSEIMSM IWKRLNDHGK181NWRHVYKAMT LMEYLIKTGS ERVSQQCKEN MYAVQTLKDF QYVDRDGKDQ GVNVREKAKQ241LVALLRDEDR LREERAHALK TKEKLAQTAT ASSAAVGSGP PPEAEQAWPQ SSGEEELQLQ301LALAMSKEEA DQEERIRRGD DLRLQMAIEE SKRETGGKEE SSLMDLADVF TAPAPAPTTD361PWGGPAPMAA AVPTAAPTSD PWGGPPVPPA ADPWGGPAPT PASGDPWRPA APAGPSVDPW421GGTPAPAAGE GPTPDPWGSS DGGVPVSGPS ASDPWTPAPA FSDPWGGSPA KPSTNGTTAA481GGFDTEPDEF SDFDRLRTAL PTSGSSAGEL ELLAGEVPAR SPGAFDMSGV RGSLAEAVGS541PPPAATPTPT PPTRKTPESF LGPNAALVDL DSLVSRPGPT PPGAKASNPF LPGGGPATGP601SVTNPFQPAP PATLTLNQLR LSPVPPVPGA PPTYISPLGG GPGLPPMMPP GPPAPNTNPF661LLTRTRPLEQKLISEEDLAANDILDYKDDDDKV
662 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.
