TIA-1 Antibody (clone TIA-1)
Catalog Number: LS-C312188-0.1
0.1 mL / $355.00
Antibody: Mouse Monoclonal
Applications: Immunohistochemistry, Frozen, Immunohistochemistry, Paraffin
Reactivity: Human
Format: Liquid
Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
Epsin 1 / EPN1 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G114304-100
Catalog Number: LS-G114304
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human Epsin 1(EPN1) (NM_013333), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- Epsin-1 | Epsin 1 | EPS-15-interacting protein 1 | EPN1
- Origin Species
- Human
- NCBI GenBank
- NM_013333
- SwissProt
- Q9Y6I3
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 57.3 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MSTSSLRRQM KNIVHNYSEA EIKVREATSN DPWGPSSSLM SEIADLTYNV VAFSEIMSMI61WKRLNDHGKN WRHVYKAMTL MEYLIKTGSE RVSQQCKENM YAVQTLKDFQ YVDRDGKDQG121VNVREKAKQL VALLRDEDRL REERAHALKT KEKLAQTATA SSAAVGSGPP PEAEQAWPQS181SGEEELQLQL ALAMSKEEAD QEERIRRGDD LRLQMAIEES KRETGGKEES SLMDLADVFT241APAPAPTTDP WGGPAPMAAA VPTAAPTSDP WGGPPVPPAA DPWGGPAPTP ASGDPWRPAA301PAGPSVDPWG GTPAPAAGEG PTPDPWGSSD GGVPVSGPSA SDPWTPAPAF SDPWGGSPAK361PSTNGTTAGG FDTEPDEFSD FDRLRTALPT SGSSAGELEL LAGEVPARSP GAFDMSGVRG421SLAEAVGSPP PAATPTPTPP TRKTPESFLG PNAALVDLDS LVSRPGPTPP GAKASNPFLP481GGGPATGPSV TNPFQPAPPA TLTLNQLRLS PVPPVPGAPP TYISPLGGGP GLPPMMPPGP541PAPNTNPFLLTRTRPLEQKLISEEDLAANDILDYKDDDDKV
550 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.
