Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
ELF2 / NERF Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G115017-100
Catalog Number: LS-G115017
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human ELF2 (NM_006874), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- E74-like factor 2 | ELF2 | EU32 | NERF-1A | New ETS-related factor | NERF | NERF-1B | NERF-1a | b | NERF-2
- Origin Species
- Human
- NCBI GenBank
- NM_006874
- SwissProt
- Q15723
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 57.2 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MATSLHEGPT NQLDLLIRAV EASVHSSNAH CTDKTIEAAE ALLHMESPTC LRDSRSPVEV61FVPPCVSTPE FIHAAMRPDV ITETVVEVST EESEPMDTSP IPTSPDSHEP MKKKKVGRKP121KTQQSPISNG SPELGIKKKP REGKGNTTYL WEFLLDLLQD KNTCPRYIKW TQREKGIFKL181VDSKAVSKLW GKHKNKPDMN YETMGRALRY YYQRGILAKV EGQRLVYQFK DMPKNIVVID241DDKSETCNED LAGTTDEKSL ERVSLSAESL LKAASSVRSG KNSSPINCSR AEKGVARVVN301ITSPGHDASS RSPTTTASVS ATAAPRTVRV AMQVPVVMTS LGQKISTVAV QSVNAGAPLI361TSTSPTTATS PKVVIQTIPT VMPASTENGD KITMQPAKII TIPATQLAQC QLQTKSNLTG421SGSINIVGTP LAVRALTPVS IAHGTPVMRL SMPTQQASGQ TPPRVISAVI KGPEVKSEAV481AKKQEHDVKT LQLVEEKPAD GNKTVTHVVV VSAPSAIALP VTMKTEGLVT CEKTRTRPLEQKLISEEDLAANDILDYKDDDDKV
533 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.