Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
ELF2 / NERF Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G102592-100
Catalog Number: LS-G102592
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human ELF2 (NM_201999), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- E74-like factor 2 | ELF2 | EU32 | NERF-1A | New ETS-related factor | NERF | NERF-1B | NERF-1a | b | NERF-2
- Origin Species
- Human
- NCBI GenBank
- NM_006874
- SwissProt
- Q15723
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 62.5 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MTSAVVDSGG TILELSSNGV ENQEESEKVS EYPAVIVEPV PSARLEQGYA AQVLVYDDET61YMMQDVAEEQ EVETENVETV EASVHSSNAH CTDKTIEAAE ALLHMESPTC LRDSRSPEFI121HAAMRPDVIT ETVVEVSTEE SEPMDTSPIP TSPDSHEPMK KKKVGRKPKT QQSPISNGSP181ELGIKKKPRE GKGNTTYLWE FLLDLLQDKN TCPRYIKWTQ REKGIFKLVD SKAVSKLWGK241HKNKPDMNYE TMGRALRYYY QRGILAKVEG QRLVYQFKDM PKNIVVIDDD KSETCNEDLA301GTTDEKSLER VSLSAESLLK AASSVRSGKN SSPINCSRAE KGVARVVNIT SPGHDASSRS361PTTTASVSAT AAPRTVRVAM QVPVVMTSLG QKISTVAVQS VNAGAPLITS TSPTTATSPK421VVIQTIPTVM PASTENGDKI TMQPAKIITI PATQLAQCQL QTKSNLTGSG SINIVGTPLA481VRALTPVSIA HGTPVMRLSM PTQQASGQTP PRVISAVIKG PEVKSEAVAK KQEHDVKTLQ541LVEEKPADGN KTVTHVVVVS APSAIALPVT MKTEGLVTCE KTRTRPLEQKLISEEDLAANDILDYKDDDDKV
581 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.