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ECT2 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

ECT2 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all ECT2 antibodies →
$504.00each
Catalog Number: LS-G111989-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human ECT2 (NM_018098), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
ARHGEF31 | ECT2 | Protein ECT2
Origin Species
Human
NCBI GenBank
NM_018098
SwissProt
Q9H8V3

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
99.9 kDa
Protein Type
Lysate
Amino Acid Sequence
1MAENSVLTST TGRTSLADSS IFDSKVTEIS KENLLIGSTS YVEEEMPQIE TRVILVQEAG
61KQEELIKALK DIKVGFVKME SVEEFEGLDS PEFENVFVVT DFQDSVFNDL YKADCRVIGP
121PVVLNCSQKG EPLPFSCRPL YCTSMMNLVL CFTGFRKKEE LVRLVTLVHH MGGVIRKDFN
181SKVTHLVANC TQGEKFRVAV SLGTPIMKPE WIYKAWERRN EQDFYAAVDD FRNEFKVPPF
241QDCILSFLGF SDEEKTNMEE MTEMQGGKYL PLGDERCTHL VVEENIVKDL PFEPSKKLYV
301VKQEWFWGSI QMDARAGETM YLYEKANTPE LKKSVSMLSL NTPNSNRKRR RLKETLAQLS
361RETDVSPFPP RKRPSAEHSL SIGSLLDISN TPESSINYGD TPKSCTKSSK SSTPVPSKQS
421ARWQVAKELY QTESNYVNIL ATIIQLFQVP LEEEGQRGGP ILAPEEIKTI FGSIPDIFDV
481HTKIKDDLED LIVNWDESKS IGDIFLKYSK DLVKTYPPFV NFFEMSKETI IKCEKQKPRF
541HAFLKINQAK PECGRQSLVE LLIRPVQRLP SVALLLNDLK KHTADENPDK STLEKAIGSL
601KEVMTHINED KRKTEAQKQI FDVVYEVDGC PANLLSSHRS LVQRVETISL GEHPCDRGEQ
661VTLFLFNDCL EIARKRHKVI GTFRSPHGQT RPPASLKHIH LMPLSQIKKV LDIRETEDCH
721NAFALLVRPP TEQANVLLSF QMTSDELPKE NWLKMLCRHV ANTICKADAE NLIYTADPES
781FEVNTKDMDS TLSRASRAIK KTSKKVTRAF SFSKTPKRAL RRALMTSHGS VEGRSPSSND
841KHVMSRLSST SSLAGIPSPS LVSLPSFFER RSHTLSRSTT HLI
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

883 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.