Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
E6AP / UBE3A Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G107598-100
Catalog Number: LS-G107598
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human UBE3A (NM_130839), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- ANCR | E6-ap isoform-III | E6AP ubiquitin-protein ligase | EPVE6AP | HPVE6A | AS | UBE3A | CTCL tumor antigen se37-2 | E6-AP | E6AP | Ubiquitin protein ligase E3A | Ubiquitin-protein ligase E3A
- Origin Species
- Human
- NCBI GenBank
- NM_000462
- SwissProt
- Q05086
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 100.1 kda
- Protein Type
- Lysate
- Amino Acid Sequence
1MATACKRSGE PQSDDIEASR MKRAAAKHLI ERYYHQLTEG CGNEACTNEF CASCPTFLRM61DNNAAAIKAL ELYKINAKLC DPHPSKKGAS SAYLENSKGA PNNSCSEIKM NKKGARIDFK121DVTYLTEEKV YEILELCRER EDYSPLIRVI GRVFSSAEAL VQSFRKVKQH TKEELKSLQA181KDEDKDEDEK EKAACSAAAM EEDSEASSSR IGDSSQGDNN LQKLGPDDVS VDIDAIRRVY241TRLLSNEKIE TAFLNALVYL SPNVECDLTY HNVYSRDPNY LNLFIIVMEN RNLHSPEYLE301MALPLFCKAM SKLPLAAQGK LIRLWSKYNA DQIRRMMETF QQLITYKVIS NEFNSRNLVN361DDDAIVAASK CLKMVYYANV VGGEVDTNHN EEDDEEPIPE SSELTLQELL GEERRNKKGP421RVDPLETELG VKTLDCRKPL IPFEEFINEP LNEVLEMDKD YTFFKVETEN KFSFMTCPFI481LNAVTKNLGL YYDNRIRMYS ERRITVLYSL VQGQQLNPYL RLKVRRDHII DDALVRLEMI541AMENPADLKK QLYVEFEGEQ GVDEGGVSKE FFQLVVEEIF NPDIGMFTYD ESTKLFWFNP601SSFETEGQFT LIGIVLGLAI YNNCILDVHF PMVVYRKLMG KKGTFRDLGD SHPVLYQSLK661DLLEYEGNVE DDMMITFQIS QTDLFGNPMM YDLKENGDKI PITNENRKEF VNLYSDYILN721KSVEKQFKAF RRGFHMVTNE SPLKYLFRPE EIELLICGSR NLDFQALEET TEYDGGYTRD781SVLIREFWEI VHSFTDEQKR LFLQFTTGTD RAPVGGLGKL KMIIAKNGPD TERLPTSHTC841FNVLLLPEYS SKEKLKERLL KAITYAKGFG MLTRTRPLEQKLISEEDLAANDILDYKDDDDKV
872 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.