Detection of Human and Mouse E6AP by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells. Antibodies: Affinity purified rabbit anti-E6AP antibody used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
E6AP / UBE3A Antibody (aa75-125)
Primary AntibodyLSBio Guarantee
| Antibody: | Rabbit Polyclonal |
| Applications: | Immunohistochemistry (general), Immunoprecipitation, Western Blot |
| Reactivity: | Human, Mouse |
| Format: | Liquid |
$301.00each
Catalog Number: LS-C287248-10
Catalog Number: LS-C287248
For bulk orders or custom formulations:
Request a QuoteOverview
- Description
- UBE3A antibody LS-C287248 is an unconjugated rabbit polyclonal antibody to UBE3A (E6AP) (aa75-125) from human. It is reactive with human and mouse. Validated for IP and WB.
- Application
- IHC, IP, WB
- Reactivity
- Hu, Ms
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Alternative Names
- ANCR | E6-ap isoform-III | E6AP ubiquitin-protein ligase | EPVE6AP | HPVE6A | AS | UBE3A | CTCL tumor antigen se37-2 | E6-AP | E6AP | Ubiquitin protein ligase E3A | Ubiquitin-protein ligase E3A
- Recommended Dilution: WB
- 1:1000 - 1:5000
- UniProt Number
- Q05086
- NCBI GenBank
- NM_000462 | NP_000453.2
- SwissProt
- Q05086
Target
- Target
- Human E6AP / UBE3A
- Antigen Species
- Human
- Epitope
- Region between residue 75 and 125 of human Ubiquitin Protein Ligase E3A using the numbering given in entry NP_000453.2 (GeneID 7337).
- Gene Name
- UBE3A
Antibody
- Host Species
- Rabbit
- Clonality
- Polyclonal
- Isotype
- IgG
Immunogen
- Immunogen
- A portion of GeneID: 7337 corresponding to amino acids between 75 and 125 of UniprotID: Q05086
- Immunogen Type
- Recombinant Protein Fragment
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- Tris-citrate/phosphate buffer, pH 7-8, 0.09% sodium azide.
- Preservative
- Yes
- Purification Method
- Affinity Purified
Storage & Handling
- Storage Conditions
- Store at 2-8°C for up to one year.
- Recommended Storage Buffer
- Tris-citrate