Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
DTNB / Dystrobrevin Beta Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G104016-100
Catalog Number: LS-G104016
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human Dystrobrevin beta(DTNB) (NM_183361), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- Beta-dystrobrevin | DTNB | Dystrobrevin beta | Dystrobrevin | beta | DTN-B | Dystrobrevin b dtn-b1
- Origin Species
- Human
- NCBI GenBank
- NM_021907
- SwissProt
- O60941
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 63.6 kda
- Protein Type
- Lysate
- Amino Acid Sequence
1MIEESGNKRK TMAEKRQLFI EMRAQNFDVI RLSTYRTACK LRFVQKRCNL HLVDIWNMIE61AFRDNGLNTL DHTTEISVSR LETVISSIYY QLNKRLPSTH QISVEQSISL LLNFMIAAYD121SEGRGKLTVF SVKAMLATMC GGKMLDKLRY VFSQMSDSNG LMIFSKFDQF LKEVLKLPTA181VFEGPSFGYT EHSVRTCFPQ QRKIMLNMFL DTMMADPPPQ CLVWLPLMHR LAHVENVFHP241VECSYCRCES MMGFRYRCQQ CHNYQLCQNC FWRGHAGGPH SNQHQMKEHS SWKSPAKKLS301HAISKSLGCV PTREPPHPVF PEQPEKPLDL AHIVPPRPLT NMNDTMVSHM SSGVPTPTKS361VLDSPSRLDE EHRLIARYAA RLAAEAGNVT RPPTDLSFNF DANKQQRQLI AELENKNREI421LQEIQRLRLE HEQASQPTPE KAQQNPTLLA ELRLLRQRKD ELEQRMSALQ ESRRELMVQL481EELMKLLKAQ ATGSPHTSPT HGGGRPMPMP VRSTSAGSTP THCPQDSLSG VGGDVQEAFA541QAEEGAEEEE EKMQNGKDRGTRTRPLEQKLISEEDLAANDILDYKDDDDKV
560 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.