DTNB / Dystrobrevin Beta Antibody
Catalog Number: LS-C747939
2 sizes / from $281.00
Antibody: Rabbit Polyclonal
Applications: Western Blot
Reactivity: Human, Mouse
Format: Liquid
Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
Human DTNB / Dystrobrevin Beta Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293T |
| Format: | Lyophilized |
$220.00each
Catalog Number: LS-G85546-20
Catalog Number: LS-G85546
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- Description
- This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human Dystrobrevin beta(DTNB) (NM_033147) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- Beta-dystrobrevin | DTNB | Dystrobrevin beta | Dystrobrevin | beta | DTN-B | Dystrobrevin b dtn-b1
- Origin Species
- Human
- NCBI GenBank
- NM_021907
- SwissProt
- O60941
Protein
- Expression System
- Human Embryonic Kidney 293T
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 67.9 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MIEESGNKRK TMAEKRQLFI EMRAQNFDVI RLSTYRTACK LRFVQKRCNL HLVDIWNMIE61AFRDNGLNTL DHTTEISVSR LETVISSIYY QLNKRLPSTH QISVEQSISL LLNFMIAAYD121SEGRGKLTVF SVKAMLATMC GGKMLDKLRY VFSQMSDSNG LMIFSKFDQF LKEVLKLPTA181VFEGPSFGYT EHSVRTCFPQ QRKIMLNMFL DTMMADPPPQ CLVWLPLMHR LAHVENVFHP241VECSYCRCES MMGFRYRCQQ CHNYQLCQNC FWRGHAGGPH SNQHQMKEHS SWKSPAKKLS301HAISKSLGCV PTREPPHPVF PEQPEKPLDL AHIVPPRPLT NMNDTMVSHM SSGVPTPTKR361LQYSQDIPSH LADEHALIAS YVARLQHCAR VLDSPSRLDE EHRLIARYAA RLAAEAGNVT421RPPTDLSFNF DANKQQRQLI AELENKNREI LQEIQRLRLE HEQASQPTPE KAQQNPTLLA481ELRLLRQRKD ELEQRMSALQ ESRRELMVQL EELMKLLKEE EQKQAAQATG SPHTSPTHGG541GRPMPMPVRS TSAGSTPTHC PQDSLSGVGG DVQEAFAQAE EGAEEEEEKM QNGKDRGTRTRPLEQKLISEEDLAANDILDYKDDDDKV
597 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Lyophilized
- Formulation
- Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4
Storage & Handling
- Storage Conditions
- Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.
