Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
DPP6 / Dipeptidylpeptidase 6 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G120584-100
Catalog Number: LS-G120584
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human DPP6 (NM_001039350), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- Dipeptidylpeptidase VI | DPP6 | Dipeptidyl peptidase 6 | Dipeptidyl peptidase VI | Dipeptidyl-peptidase 6 | Dipeptidylpeptidase 6 | DPP VI | DPPX | VF2 | Dipeptidyl aminopeptidase-like protein 6
- Origin Species
- Human
- NCBI GenBank
- NM_001936
- SwissProt
- P42658
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 91.1 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MKEKAMIKTA KMQGNVMELV GSNPPQRNWK GIAIALLVIL VICSLIVTSV ILLTPAEDNS61LSQKKKVTVE DLFSEDFKIH DPEAKWISDT EFIYREQKGT VRLWNVETNT STVLIEGKKI121ESLRAIRYEI SPDREYALFS YNVEPIYQHS YTGYYVLSKI PHGDPQSLDP PEVSNAKLQY181AGWGPKGQQL IFIFENNIYY CAHVGKQAIR VVSTGKEGVI YNGLSDWLYE EEILKTHIAH241WWSPDGTRLA YAAINDSRVP IMELPTYTGS IYPTVKPYHY PKAGSENPSI SLHVIGLNGP301THDLEMMPPD DPRMREYYIT MVKWATSTKV AVTWLNRAQN VSILTLCDAT TGVCTKKHED361ESEAWLHRQN EEPVFSKDGR KFFFIRAIPQ GGRGKFYHIT VSSSQPNSSN DNIQSITSGD421WDVTKILAYD EKGNKIYFLS TEDLPRRRQL YSANTVGNFN RQCLSCDLVE NCTYFSASFS481HSMDFFLLKC EGPGVPMVTV HNTTDKKKMF DLETNEHVKK AINDRQMPKV EYRDIEIDDY541NLPMQILKPA TFTDTTHYPL LLVVDGTPGS QSVAEKFEVS WETVMVSSHG AVVVKCDGRG601SGFQGTKLLH EVRRRLGLLE EKDQMEAVRT MLKEQYIDRT RVAVFGKDYG GYLSTYILPA661KGENQGQTFT CGSALSPITD FKLYASAFSE RYLGLHGLDN RAYEMTKVAH RVSALEEQQF721LIIHPTADEK IHFQHTAELI TQLIRGKANY SLQIYPDESH YFTSSSLKQH LYRSIINFFV781ECFRIQDKLL TVTAKEDEEE DTRTRPLEQKLISEEDLAANDILDYKDDDDKV
801 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.