Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
DPP6 / Dipeptidylpeptidase 6 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G118292-100
Catalog Number: LS-G118292
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human DPP6 (NM_001936), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- Dipeptidylpeptidase VI | DPP6 | Dipeptidyl peptidase 6 | Dipeptidyl peptidase VI | Dipeptidyl-peptidase 6 | Dipeptidylpeptidase 6 | DPP VI | DPPX | VF2 | Dipeptidyl aminopeptidase-like protein 6
- Origin Species
- Human
- NCBI GenBank
- NM_001936
- SwissProt
- P42658
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 91.2 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MTTAKEPSAS GKSVQQQEQE LVGSNPPQRN WKGIAIALLV ILVICSLIVT SVILLTPAED61NSLSQKKKVT VEDLFSEDFK IHDPEAKWIS DTEFIYREQK GTVRLWNVET NTSTVLIEGK121KIESLRAIRY EISPDREYAL FSYNVEPIYQ HSYTGYYVLS KIPHGDPQSL DPPEVSNAKL181QYAGWGPKGQ QLIFIFENNI YYCAHVGKQA IRVVSTGKEG VIYNGLSDWL YEEEILKTHI241AHWWSPDGTR LAYAAINDSR VPIMELPTYT GSIYPTVKPY HYPKAGSENP SISLHVIGLN301GPTHDLEMMP PDDPRMREYY ITMVKWATST KVAVTWLNRA QNVSILTLCD ATTGVCTKKH361EDESEAWLHR QNEEPVFSKD GRKFFFIRAI PQGGRGKFYH ITVSSSQPNS SNDNIQSITS421GDWDVTKILA YDEKGNKIYF LSTEDLPRRR QLYSANTVGN FNRQCLSCDL VENCTYFSAS481FSHSMDFFLL KCEGPGVPMV TVHNTTDKKK MFDLETNEHV KKAINDRQMP KVEYRDIEID541DYNLPMQILK PATFTDTTHY PLLLVVDGTP GSQSVAEKFE VSWETVMVSS HGAVVVKCDG601RGSGFQGTKL LHEVRRRLGL LEEKDQMEAV RTMLKEQYID RTRVAVFGKD YGGYLSTYIL661PAKGENQGQT FTCGSALSPI TDFKLYASAF SERYLGLHGL DNRAYEMTKV AHRVSALEEQ721QFLIIHPTAD EKIHFQHTAE LITQLIRGKA NYSLQIYPDE SHYFTSSSLK QHLYRSIINF781FVECFRIQDK LLTVTAKEDE EEDTRTRPLEQKLISEEDLAANDILDYKDDDDKV
803 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.