Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
DLG4 / PSD95 Protein (Over-Expression Lysate Myc-DDK (Flag))
ProteinLSBio Guarantee
| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G118628-100
Catalog Number: LS-G118628
For bulk orders or custom formulations:
Request a QuoteOverview
- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human PSD95(DLG4) (NM_001365), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- Disks large homolog 4 | Presynaptic density protein 95 | PSD95 | SAP-90 | Synapse-associated protein 90 | PSD-95 | SAP90 | Tax interaction protein 15 | Discs large homolog 4 | DLG4
- Origin Species
- Human
- NCBI GenBank
- NM_001365
- SwissProt
- P78352
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 85.2 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MSQRPRAPRS ALWLLAPPLL RWAPPLLTVL HSDLFQALLD ILDYYEASLS ESQKYRYQDE61DTPPLEHSPA HLPNQANSPP VIVNTDTLEA PGYELQVNGT EGEMEYEEIT LERGNSGLGF121SIAGGTDNPH IGDDPSIFIT KIIPGGAAAQ DGRLRVNDSI LFVNEVDVRE VTHSAAVEAL181KEAGSIVRLY VMRRKPPAEK VMEIKLIKGP KGLGFSIAGG VGNQHIPGDN SIYVTKIIEG241GAAHKDGRLQ IGDKILAVNS VGLEDVMHED AVAALKNTYD VVYLKVAKPS NAYLSDSYAP301PDITTSYSQH LDNEISHSSY LGTDYPTAMT PTSPRRYSPV AKDLLGEEDI PREPRRIVIH361RGSTGLGFNI VGGEDGEGIF ISFILAGGPA DLSGELRKGD QILSVNGVDL RNASHEQAAI421ALKNAGQTVT IIAQYKPEEY SRFEAKIHDL REQLMNSSLG SGTASLRSNP KRGFYIRALF481DYDKTKDCGF LSQALSFRFG DVLHVIDASD EEWWQARRVH SDSETDDIGF IPSKRRVERR541EWSRLKAKDW GSSSGSQGRE DSVLSYETVT QMEVHYARPI IILGPTKDRA NDDLLSEFPD601KFGSCVPHTT RPKREYEIDG RDYHFVSSRE KMEKDIQAHK FIEAGQYNSH LYGTSVQSVR661EVAEQGKHCI LDVSANAVRR LQAAHLHPIA IFIRPRSLEN VLEINKRITE EQARKAFDRA721TKLEQEFTEC FSAIVEGDSF EEIYHKVKRV IEDLSGPYIW VPARERLTRTRPLEQKLISEEDLAANDILDYKDDDDKV
767 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.