ICC of PSD95 in cultures of dissociated rat hippocampal neurons. Fixation: Cold 4% paraformaldehyde/0.2% glutaraldehyde in 0.1M sodium phosphate buffer. Primary Antibody: Mouse Anti-PSD95 at 1:1000 for 12 hours at 4°C. Secondary Antibody: FITC Goat Anti-Mouse IgG (green) at 1:50 for 30 minutes at RT. Experiment performed with the unconjugated form of the antibody. Other forms have not been tested.
DLG4 / PSD95 Antibody (clone 6G6)
Primary AntibodyLSBio Guarantee
| Antibody: | Mouse Monoclonal |
| Applications: | Immunocytochemistry, Immunofluorescence, Immunohistochemistry (general), Western Blot |
| Reactivity: | Bovine, Human, Mouse, Rat |
| Format: | Liquid |
$319.00each
Catalog Number: LS-C150376-25
Catalog Number: LS-C150376
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- Description
- PSD95 antibody LS-C150376 is an unconjugated mouse monoclonal antibody to PSD95 (DLG4) from rat. It is reactive with human, mouse, rat and other species. Validated for ICC, IF, IHC and WB.
- Application
- ICC, IF, IHC, WB
- Reactivity
- Bov, Hu, Ms, Rt
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Monoclonal Antibodies
- Alternative Names
- Disks large homolog 4 | Presynaptic density protein 95 | PSD95 | SAP-90 | Synapse-associated protein 90 | PSD-95 | SAP90 | Tax interaction protein 15 | Discs large homolog 4 | DLG4
- Recommended Dilution: WB
- 1:250
- UniProt Number
- P78352
- NCBI GenBank
- NM_001365 | NP_001356.1
- SwissProt
- P78352
Target
- Target
- Rat DLG4 / PSD95
- Antigen Species
- Rat
- Epitope
- Detects an ~100kDa protein corresponding to the apparent molecular mass of PSD-95 on SDS-PAGE immunoblots. An additional protein of >100 kDa is also detected. Additional cross-reactive bands are detected at ~75 kDa and 50 kDa in rat and mouse samples.
- Gene Name
- DLG4
Antibody
- Host Species
- Mouse
- Clonality
- Monoclonal
- Clone
- 6G6
- Isotype
- IgG2a
Immunogen
- Immunogen
- Recombinant rat PSD-95.
- Immunogen Type
- Recombinant Immunogen
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- PBS, pH 7.4, 0.09% Sodium Azide, 50% Glycerol
- Concentration
- 1 mg/mL
- Preservative
- Yes
- Purification Method
- Protein G Affinity
Storage & Handling
- Storage Conditions
- Store at -20°C for up to 1 year.
- Recommended Storage Buffer
- PBS