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Dipeptidylpeptidase 10 / DPP10 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

Dipeptidylpeptidase 10 / DPP10 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$504.00each
Catalog Number: LS-G110922-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human DPP10 (NM_020868), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
Dipeptidyl peptidase X | Dipeptidyl-peptidase 10 | Dipeptidylpeptidase 10 | DPL2 | DPRP-3 | DPRP3 | Dipeptidyl peptidase 10 | DPP10 | DPPY | KIAA1492 | DPP X
Origin Species
Human
NCBI GenBank
NM_020868
SwissProt
Q8N608

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
90.7 kDa
Protein Type
Lysate
Amino Acid Sequence
1MNQTASVSHH IKCQPSKTIK ELGSNSPPQR NWKGIAIALL VILVVCSLIT MSVILLTPDE
61LTNSSETRLS LEDLFRKDFV LHDPEARWIN DTDVVYKSEN GHVIKLNIET NATTLLLENT
121TFVTFKASRH SVSPDLKYVL LAYDVKQIFH YSYTASYVIY NIHTREVWEL NPPEVEDSVL
181QYAAWGVQGQ QLIYIFENNI YYQPDIKSSS LRLTSSGKEE IIFNGIADWL YEEELLHSHI
241AHWWSPDGER LAFLMINDSL VPTMVIPRFT GALYPKGKQY PYPKAGQMNP TIKLYVVNLY
301GPTHTLELMP PDSFKSREYY ITMVKWVSNT KTVVRWLNRA QNISILTVCE TTTGACSKKY
361EMTSDTWLSQ QNEEPVFSRD GSKFFMTVPV KQGGRGEFHH IAMFLIQSKS EQITVRHLTS
421GNWEVIKILA YDETTQKIYF LSTESSPRGR QLYSASTEGL LNRQCISCNF MKEQCTYFDA
481SFSPMNQHFL LFCEGPRVPV VSLHSTDNPA KYFILESNSM LKEAILKKKI GKPEIKILHI
541DDYELPLQLS LPKDFMDRNQ YALLLIMDEE PGGQLVTDKF HIDWDSVLID MDNVIVARFD
601GRGSGFQGLK ILQEIHRRLG SVEVKDQITA VKFLLKLPYI DSKRLSIFGK GYGGYIASMI
661LKSDEKLFKC GSVVAPITDL KLYASAFSER YLGMPSKEES TYQAASVLHN VHGLKEENIL
721IIHGTADTKV HFQHSAELIK HLIKAGVNYT MQVYPDEGHN VSEKSKYHLY STILKFFSDC
781LKEEISVLPQ EPEEDE
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

796 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.