Human Apolipoprotein H Antibody (IgG) ELISA Kit (Sandwich ELISA)
Catalog Number: LS-F67372-1
1 plate / $591.00
Reactivity: Human
Detection Method Colorimetric
Format: 96-Well Strip Plate
Anti IL-10 whole antiserum was tested against recombinant IL10. Recombinant IL-10 was run on a 4-20% reducing gel, and transferred to nitrocellulose. Membrane was blocked in 3% BSA-TBS for 1 Hour 4oC and probed with p/n 109-401-312 diluted 1:200 in 3% BSA-TBS o/n 4oC. Primary antibody was detected with Gt anti Rb 611-103-122 Lot#21231 1:20,000 in MB-070 1 Hour 4oC using FemtoMax Super sensitive chemiluminescent substrate. Blot was imaged with VersaDoc Imaging system by Biorad. Other detection systems will yield similar results.
IL-10 Antibody
Primary AntibodyLSBio Guarantee
| Antibody: | Rabbit Polyclonal |
| Applications: | Enzyme-Linked Immunosorbent Assay, Western Blot |
| Reactivity: | Human |
| Format: | Liquid |
$579.00each
Catalog Number: LS-C744648-100
Catalog Number: LS-C744648
For bulk orders or custom formulations:
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- Description
- IL-10 antibody LS-C744648 is an unconjugated rabbit polyclonal antibody to human IL-10. Validated for ELISA and WB.
- Application
- ELISA, WB
- Reactivity
- Hu
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Alternative Names
- CSIF | GVHDS | IL-10 | IL10 | IL10A | Interleukin-10 | Interleukin 10 | TGIF
- Recommended Dilution: ELISA
- 1:20000 - 1:100000
- Recommended Dilution: WB
- 1:2000 - 1:10000
- UniProt Number
- P22301
- NCBI GenBank
- NM_000572 | NP_000563.1
- SwissProt
- P22301
Target
- Target
- Human IL-10
- Antigen Species
- Human
- Epitope
- The antiserum detects recombinant and native IL-10 present in body fluids and cell supernatants in various assays (ie. IL-1 stimulated IL-10 production from fibroblasts). The antibody recognizes hu IL-10. The antibody will not neutralize the biological activity of murine IL-10.
- Gene Name
- IL10
Antibody
- Host Species
- Rabbit
- Clonality
- Polyclonal
- Origin Species
- Human
Immunogen
- Immunogen
- The whole rabbit serum was prepared by repeated immunizations with human IL-10.
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- Antiserum, 0.01% Sodium Azide
- Concentration
- 85 mg/mL
- Volume
- 100 Microliter
- Preservative
- Yes
- Purification Method
- Unpurified (Serum / Ascites / Supernatant)
Storage & Handling
- Storage Conditions
- Store vial at -20°C or below prior to opening. Dilute 1:10 to minimize loss. Store the vial at -20°C or below after dilution. Avoid freeze-thaw cycles.
