Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
DCTN4 / Dynactin 4 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G125487-100
Catalog Number: LS-G125487
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human DCTN4 (NM_001135643), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- Dynactin subunit 4 | DYN4 | Dynactin 4 | Dynactin p62 | Dynactin 4 (p62) | Dynactin p62 subunit | p62 | DCTN4 | Dynactin subunit p62
- Origin Species
- Human
- NCBI GenBank
- NM_016221
- SwissProt
- Q9UJW0
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 53 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MASLLQSDRV LYLVQGEKKV RAPLSQLYFC RYCSELRSLE CVSHEVDSHY CPSCLENMPS61AEAKLKKNRC ANCFDCPGCM HTLSTRATSI STQLPDDPAK TTMKKAYYLA CGFCRWTSRD121VGMADKSVAS GGWQEPENPH TQRMNKLIEY YQQLAQKEKV ERDRKKLARR RNYMPLAFSQ181HTIHVVDKYG LGTRLQRPRA GASISTLAGL SLKEGEDQKE IKIEPAQAVD EVEPLPEDYY241TRPVNLTEVT TLQQRLLQPD FQPVCASQLY PRHKHLLIKR SLRCRKCEHN LSKPEFNPTS301IKFKIQLVAV NYIPEVRIMS IPNLRYMKES QVLLTLTNPV ENLTHVTLFE CEEGDPDDIN361STAKVVVPPK ELVLAGKDAA AEYDELAEPQ DFQDDPDIIA FRKANKVGIF IKVTPQREEG421EVTVCFKMKH DFKNLAAPIR PIEESDQGTE VIWLTQHVEL SLGPLLPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
467 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.