Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
DCTN4 / Dynactin 4 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G101411-100
Catalog Number: LS-G101411
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human DCTN4 (NM_016221), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- Dynactin subunit 4 | DYN4 | Dynactin 4 | Dynactin p62 | Dynactin 4 (p62) | Dynactin p62 subunit | p62 | DCTN4 | Dynactin subunit p62
- Origin Species
- Human
- NCBI GenBank
- NM_016221
- SwissProt
- Q9UJW0
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 52.2 kda
- Protein Type
- Lysate
- Amino Acid Sequence
1MASLLQSDRV LYLVQGEKKV RAPLSQLYFC RYCSELRSLE CVSHEVDSHY CPSCLENMPS61AEAKLKKNRC ANCFDCPGCM HTLSTRATSI STQLTDDPAK TTMKKAYYLA CGFCRWTSRD121VGMADKSVAS GGWQEPENPH TQRMNKLIEY YQQLAQKEKV ERDRKKLARR RNYMPLAFSD181KYGLGTRLQR PRAGASISTL AGLSLKEGED QKEIKIEPAQ AVDEVEPLPE DYYTRPVNLT241EVTTLQQRLL QPDFQPVCAS QLYPRHKHLL IKRSLRCRKC EHNLSKPEFN PTSIKFKIQL301VAVNYIPEVR IMSIPNLRYM KESQVLLTLT NPVENLTHVT LFECEEGDPD DINSTAKVVV361PPKELVLAGK DAAAEYDELA EPQDFQDDPD IIAFRKANKV GIFIKVTPQR EEGEVTVCFK421MKHDFKNLAA PIRPIEESDQ GTEVIWLTQH VELSLGPLLPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
460 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.