Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
CRTR1 / TFCP2L1 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G113865-100
Catalog Number: LS-G113865
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human CRTR1(TFCP2L1) (NM_014553), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- CRTR1 | LBP-9 | TFCP2L1 | CRTR-1 | LBP9 | Transcription factor LBP-9
- Origin Species
- Human
- NCBI GenBank
- NM_014553
- SwissProt
- Q9NZI6
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 54.4 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MLFWHTQPEH YNQHNSGSYL RDVLALPIFK QEEPQLSPEN EARLPPLQYV LCAATSPAVK61LHEETLTYLN QGQSYEIRLL ENRKLGDFQD LNTKYVKSII RVVFHDRRLQ YTEHQQLEGW121RWSRPGDRIL DIDIPLSVGI LDPRASPTQL NAVEFLWDPA KRASAFIQVH CISTEFTPRK181HGGEKGVPFR VQIDTFKQNE NGEYTEHLHS ASCQIKVFKP KGADRKQKTD REKMEKRTAQ241EKEKYQPSYE TTILTECSPW PDVAYQVNSA PSPSYNGSPN SFGLGEGNAS PTHPVEALPV301GSDHLLPSAS IQDAQQWLHR NRFSQFCRLF ASFSGADLLK MSRDDLVQIC GPADGIRLFN361AIKGRNVRPK MTIYVCQELE QNRVPLQQKR DGSGDSNLSV YHAIFLEELT TLELIEKIAN421LYSISPQHIH RVYRQGPTGI HVVVSNEMVQ NFQDESCFVL STIKAESNDG YHIILKCGLTRTRPLEQKLISEEDLAANDILDYKDDDDKV
479 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.